trichinellosis/albumin

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Minimizing ELISA background in the diagnosis of swine trichinellosis.

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After confirming that long-term serum storage (frozen at -20 C for greater than 3 mo) causes optical density to drift upward, several modifications of an enzyme-linked immunosorbent assay (ELISA) protocol were evaluated to identify a protocol that would reduce background in porcine sera tested for

Persistence of reactivity against the 45 k Da glycoprotein in late trichinellosis patients.

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Over the years, the opinions of clinicians on the existence of the so-called chronic trichinellosis or late sequelae of infection have differed. However, the persistence of a humoral immune response against Trichinella in these late-stage patients has been confirmed using specific tests such as the

The use of a synthetic antigen for the serological diagnosis of human trichinellosis.

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Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed,

Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis.

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Diagnostic methods for parasite infections still highly depend on the identification of the parasites by direct methods such as microscopic examination of blood, stool and tissue biopsies. Serodiagnosis is often carried out to complement the direct methods; however, few synthetic antigens with

Acute trichinellosis in children compared with adults.

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OBJECTIVE Trichinellosis is a cosmopolitan parasite infection caused by Trichinella nematodes that is acquired from consumption of raw meat from several animal species. Knowledge of the clinical pattern and laboratory features of the disease in childhood is limited. The purpose is to study the

Human trichinosis: studies on eleven cases affecting two families in Nova Scotia.

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A two-family outbreak of 11 cases of trichinosis with one fatality has been described. Efforts were made to obtain an early assessment of the severity of parasitization on the basis of initial clinical appearance, serial electrocardiograms, changes in eosinophil count, serum protein analyses and

Re-evaluation of patients involved in a trichinellosis outbreak caused by Trichinella britovi 15 years after infection.

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This study re-evaluates 13 out of 48 subjects involved in a trichinellosis outbreak that occurred in Central Italy (Umbria Region) in 1988 resulting from the consumption of raw boar meat harboring Trichinella britovi. During the outbreak, 28 of 48 serologically positive subjects were asymptomatic,

Therapeutic potential of myrrh and ivermectin against experimental Trichinella spiralis infection in mice.

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Trichinosis is a parasitic zoonosis caused by the nematode Trichinella spiralis. Anthelmintics are used to eliminate intestinal adults as well as tissue-migrating and encysted larvae. This study aimed to investigate the effects of ivermectin and myrrh obtained from the aloe-gum resin of Commiphora

Effect of Trichinella spiralis infection on passive cutaneous anaphylaxis in mice.

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Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies,

Inhibition of passive systemic anaphylaxis in guinea pigs by Trichinella spiralis infection.

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Thirty of 33 guinea pigs experimentally infected with Trichinella spiralis survived passive systemic anaphylactic reactions which were fatal for 32 of 33 uninfected controls. Guinea pigs were infected with viable excysted trichinella larvae 20 to 32 days before they and uninfected controls were

Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis.

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A micro enzyme-linked immunosorbent assay utilizing antigen dotted onto nitrocellulose filter discs (Dot-ELISA) was developed for the rapid diagnosis of visceral leishmaniasis. Leishmania donovani promastigotes applied to filter discs in volumes of 1 microliter were placed in 96-well microtiter

Therapeutic efficacy of Dormectin, Ivermectin and Levamisole against different stages of Trichinella spiralis in rats.

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OBJECTIVE Three commonly used antihelmenthic drugs including Dormectin (0.2 mg/kg), Ivermectin (0.2 mg/kg) and Levamisole (7.5 mg/kg) were examined for their antihelmenthic efficacy against experimental Trichinella spiralis infection in rats. METHODS One hundred and twenty rats were orally infected

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