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Strana 1 od 41 výsledky
Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality.
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An amaranth (Amaranthus hypochondriacus) albumin gene, encoding the 35-kDa AmA1 protein of the seed, with a high content of essential amino acids, was used in the biolistic transformation of bread wheat (Triticum aestivum L.) variety Cadenza. The transformation cassette carried the ama1 gene under
Extractability and chromatographic characterization of wheat (triticum aestivum l.) bran protein.
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About 70% of the protein for human consumption is derived from plants, with cereals as the most important source. Wheat bran protein has a more balanced amino acid profile than that of flour. We here for the first time report the amino acid, size exclusion, and SDS-PAGE profiles of bran Osborne
Mapping and proteomic analysis of albumin and globulin proteins in hexaploid wheat kernels (Triticum aestivum L.).
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Albumins and globulins of wheat endosperm represent 20% of total kernel protein. They are soluble proteins, mainly enzymes and proteins involved in cell functions. Two-dimensional gel immobiline electrophoresis (2DE) (pH 4-7) x SDS-Page revealed around 2,250 spots. Ninety percent of the spots were
Antidiabetic Activity of Triticum aestivum Seed-Based Diet on Alloxan-Induced Diabetic Rats.
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The antidiabetic activity of Triticum aestivum seed-based diet on alloxan-induced diabetic rats was investigated. Forty-eight male and female albino rats of four groups were used for this study. Rats were sacrificed on day 28 and organs of interest were excised. Triticum aestivum seed-based diet
Expression of the high molecular weight glutenin 1Ay gene from Triticum urartu in barley.
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In this study, we successfully expressed the active 1Ay subunit of Triticum urartu in barley by Agrobacterium-mediated transformation with a transformation efficiency of 19.9%. The results of SDS-PAGE revealed that the expressed proteins of 1Ay subunit were present at some grains of each of 46
Effects of an Agropyron chromosome on endosperm proteins in common wheat Triticum aestivum L.).
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An Agropyron chromosome having a gene conferring blue color on the aleurone layer of the kernel endosperm causes a 15% increase in total grain protein content when it is added to the common wheat (2n=42) complement. In contrast, there is no effect of this chromosome on total protein content if it
Grain subproteome responses to nitrogen and sulfur supply in diploid wheat Triticum monococcum ssp. monococcum.
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Wheat grain storage proteins (GSPs) make up most of the protein content of grain and determine flour end-use value. The synthesis and accumulation of GSPs depend highly on nitrogen (N) and sulfur (S) availability and it is important to understand the underlying control mechanisms. Here we studied
Assessment of genomic and species relationships in Triticum and Aegilops by PAGE and by differential staining of seed albumins and globulins.
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Endosperm protein components from common bread wheats (Triticum aestivum L.) and related species were extracted with aluminum lactate, pH 3.2, and examined by electrophoresis in the same buffer. Electrophoretic patterns of the albumins and globulins were compared to evaluate the possibility that a
Gliadin protein antigenicity and health benefitting potential of Indian bread wheat (Triticum aestivum L.) varieties.
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Health benefitting potential of twenty leading wheat varieties was assessed for grain nutritional quality status in relation to antigenic reactivity level of gluten protein fractions. Among the nutritional parameters, macronutrients viz. starch, total sugar, total protein and gluten content were
Quantitative variation in the kernel proteins among 841 accessions of Triticum dicoccoides estimated by SDS-PAGE.
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The relative proportion and amount of proteins in five defined molecular weight (MW) regions (A1=above 71,000=71K, A2=71K-49K, A3=49K-31K, A4=31K-20K, A5=20K and less) were estimated by densitometric analyses of the amount of dye bound by kernel proteins (Fullington et al. 1980) of Triticum
Identification of Differently Regulated Proteins after Fusarium graminearum Infection of Emmer (Triticum dicoccum) at Several Grain Ripening Stages.
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This study was conducted to improve the knowledge of molecular processes involved in the interaction between Fusarium graminearum and emmer in the course of grain ripening. Emmer plants were artificially inoculated with a F. graminearum spore suspension at anthesis. In the course of grain ripening
The genome specific grain proteins and the phylogenetic interrelation between Triticum L., Elytrigia Desf., Elymus L. and Agropyron Gaertn.
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Immunochemical protein studies show that nongliadins of the ethanol-soluble fraction (EF) provide the best biochemical information on genome interrelations in cereals. After electrophoresis (pH 3.2) these genome specific proteins are shown to fall within the category of α-prolamines and albumins
Implications of high-temperature events and water deficits on protein profiles in wheat (Triticum aestivum L. cv. Vinjett) grain.
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Increased climatic variability is resulting in an increase of both the frequency and the magnitude of extreme climate events. Therefore, cereals may be exposed to more than one stress event in the growing season, which may ultimately affect crop yield and quality. Here, effects are reported of
Compared use of HPLC and FZCE for cluster analysis of Triticum spp and for the identification of T. durum adulteration.
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Wheat quality criteria continually evolve in response to market pressure and consumer preference. Characterization of cereal cultivars for quality and agronomic properties, have widely shown the importance of the protein content to ensure good quality products. The aim of this work is a comparison
Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species.
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BACKGROUND
Brachypodium distachyon L. is a newly emerging model plant system for temperate cereal crop species. However, its grain protein compositions are still not clear. In the current study, we carried out a detailed proteomics and molecular genetics study on grain glutenin proteins in B.
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