Physiological Effect of Ataulfo Mango (Manguifera Indica) Beverage in Exercise

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StanjeDokončano

Sponzorji

Universidad Autonoma de Ciudad Juarez

KLINIČNO PRESKUŠANJE: NCT03182543

BioSeek: NCT03182543

Ključne besede

Povzetek

The aim of this study is to assess the antioxidant capacity of two mango base beverages (AM1, AM2), against exercise-induced oxidative stress.

Opis

Introduction. The post-consumption physiological effect of mango cv. "Ataulfo" pulp (MP) and peel (MC) in humans before exercise has been poorly evaluated.

Objective. To evaluate the physiological response, to consumption of two mango base beverages, AM1 (200g MP/ 600mL water) and AM2 (160g MP; 40g MC / 600mL water), before and after a submaximal exercise test.

Methods. The antioxidant profile and physicochemical characteristics of AM1 and AM2 were evaluated. Several cardiorespiratory, anthropometric and body composition parameters of 19 university students, prior to the consumption of AM1, AM2 and water, were evaluated in three non-consecutive sessions (self-control cases). The glycemic, lactic and antioxidant (FRAP), uric acid (AU), reduced glutathione (GSH) and lipid oxidation (TBARS) and protein carbonyls (PC) responses were evaluated in plasma before and after an incremental cycle ergometer exercise test.

Datumi

Nazadnje preverjeno:	05/31/2017
Prvič predloženo:	05/28/2017
Predviden vpis oddan:	06/06/2017
Prvič objavljeno:	06/08/2017
Zadnja posodobitev oddana:	06/06/2017
Zadnja posodobitev objavljena:	06/08/2017
Dejanski datum začetka študija:	12/31/2016
Predvideni datum primarnega zaključka:	02/27/2017
Predvideni datum zaključka študije:	04/29/2017

Stanje ali bolezen

Exercise-induced Oxidative Stress

Intervencija / zdravljenje

Dietary Supplement: AM1

Dietary Supplement: AM2

Dietary Supplement: Control

Faza

Skupine rok

Roka	Intervencija / zdravljenje
Experimental: AM1 Mango pulp beverage	Dietary Supplement: AM1 The subject consumes mango pulp beverage (200 g mango pulp + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
Experimental: AM2 Mango pulp and peel beverage	Dietary Supplement: AM2 The subject consumes mango pulp and peel beverage (160 g mango pulp + 40 g mango peel + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
Placebo Comparator: Control Control beverage	Dietary Supplement: Control The subject consumes control beverage (600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.

Merila upravičenosti

Starost, primerna za študij	17 Years Za 17 Years
Spol, upravičen do študija	Male
Sprejema zdrave prostovoljce	Da
Merila	Inclusion Criteria: - healthy male - university student - no alcohol, cigar or drugs consumption Exclusion Criteria: - no antioxidant supplement or ergogenic consumption - health issues with performing physical activity

Izid

Primarni izidni ukrepi

1. Antioxidant capacity change [0 min (before beverage intake), 9-15 min (immediately after exercise test)]

Assessed by FRAP change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.

2. Antioxidant capacity change [0 min (before beverage intake), 9-15 (immediately after exercise test)]

Assessed by uric acid concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.

3. Antioxidant capacity change [0 min (before beverage intake), 9-15 (immediately after exercise test)]

Assessed by reduced glutathione concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.

Ukrepi sekundarnega rezultata

1. Oxidative stress change [0 min (before beverage intake), 9-15 (immediately after exercise test)]

Assessed by TBARS concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.

2. Oxidative stress change [0 min (before beverage intake), 9-15 (immediately after exercise test)]

Assessed by protein carbonyls concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.

3. Lactic acid concentration change [0 min (before performing exercise test), 3 min, 6 min, 9 min, 12 min, 15 min (during exercise test)]

Assessed by lactic acid concentration change. Capillary blood samples were taken from the fingertip, using a lancet and analyzed (YSI modelo 1500, Yellow Springs, USA)

4. Postprandial glycemic change [0 min (before beverage intake), 30 min, 60 min, 90 min, 120 min (after beverage intake)]

Assessed by blood glucose concentration change. Capillary blood samples were taken from the fingertip, using a lancet, and analyzed (ReliOn Confirm/micro Test Strips ARKRAY, USA)

Physiological Effect of Ataulfo Mango (Manguifera Indica) Beverage in Exercise

Ključne besede

uric acid

glutathione

mangovec

indijski mangovec

antioksidant