[Studies on the antioxidative activity of polysaccharides from radix Saposhnikoviae].
Ključne besede
Povzetek
OBJECTIVE
To research the antioxidant activity of polysaccharides from Saposhnikovia divaricata (Turez.) Schischk.
METHODS
Polysaccharides from Saposhnikovia divaricata (Turez.) Schischk were obtained through hot water extracting and ethanol precipitating method. Using CTAB as precipitant, Saposhnikovia polysaccharide was separated into acid Saposhnikovia polysaccharide (A-SPS) and neutral Saposhnikovia polysaccharide (N-SPS). The total reducing power, scavenging effect to superoixide anion (O2-*), hydroxyl radical (*OH), 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH*) and inhibiting effect to lipid-peroxidation induced by Fe2+ of different Saposhnikovia polysaccharides were measured in vitro chemical simulated systems for evaluating their antioxidant activity.
RESULTS
Saposhnikovia polysaccharides had some functions of scavenging free radicals and inhibiting lipid peroxidation, and scavenging effects on DPPH* and OH* were especially significant. A-SPS had better effect among different Saposhnikovia polysaccharides, and the scavenging rate of DPPH* and OH* could approach to 70% when the experimental concentration was 8 mg/ml .
CONCLUSIONS
A-SPS, potential main active ingredient of Saposhnikovia polysaccharide, has strong antioxidant activity and can be applied as a potential natural antioxidant in food and medicine industry.
