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7 rezultatov
The crystal structure of progesterone 5beta-reductase from Digitalis lanata defines a novel class of short chain dehydrogenases/reductases.
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Prijava / prijava
Progesterone 5beta-reductase (5beta-POR) catalyzes the stereospecific reduction of progesterone to 5beta-pregnane-3,20-dione and is a key enzyme in the biosynthetic pathway of cardenolides in Digitalis (foxglove) plants. Sequence considerations suggested that 5beta-POR is a member of the short chain
The measurement of digitoxin in human serum by radioimmunoassay.
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Prijava / prijava
A sensitive, specific, and relatively simple immunoassay permitting measurement of pharmacological levels of digitoxin in human serum has been developed. The assay involves binding of (125)I-labeled tyrosine-digitoxigenin (specific activity > 400 mc/mg) by rabbit antibody to digitoxin.
Interaction of Na,K-ATPase catalytic subunit with cellular proteins and other endogenous regulators.
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Prijava / prijava
Some mechanisms of regulation of Na,K-ATPase activity in various tissues including the phosphorylation of the catalytic subunit of the enzyme by different protein kinases (PKA, PKC, and tyrosine kinase) and the interaction of the alpha-subunit with different proteins (Na,K-ATPase beta- and
The Na/K-ATPase-mediated signal transduction as a target for new drug development.
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Prijava / prijava
The Na/K-ATPase, or Na+ pump, is a member of the P-type ATPase superfamily. In addition to pumping ions, the Na/K-ATPase is a receptor that not only regulates the function of protein kinases, but also acts as a scaffold, capable of tethering different proteins into a signalplex. The signaling
Growth and in vitro protein synthesis in two strains of chicks.
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Prijava / prijava
An in vitro method was used to estimate rates of skeletal muscle protein synthesis and degradation in fast (broiler) and slow (White Leghorn) growing birds. Four birds/strain were sacrificed at weekly intervals for 8 wk and biweekly thereafter to wk 14. The extensor digitalis communis, a muscle
Vein Patterning 1-encoded progesterone 5β-reductase: activity-guided improvement of catalytic efficiency.
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Prijava / prijava
Progesterone 5β-reductases (P5βR; EC 1.3.99.6) encoded by Vein Patterning 1 (VEP1) genes are capable of reducing the CC double-bond of a variety of enones enantioselectively. Sequence and activity data of orthologous P5βRs were used to define a set of residues possibly responsible for the large
Therapeutic concentrations of digitoxin inhibit endothelial focal adhesion kinase and angiogenesis induced by different growth factors.
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Prijava / prijava
OBJECTIVE
Cardiac glycosides are Na+ /K+ -ATPases inhibitors used to treat congestive heart failure and cardiac arrhythmias. Epidemiological studies indicate that patients on digitalis therapy are more protected from cancer. Evidence of a selective cytotoxicity against cancer cells has suggested
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