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Stran 1 iz 226 rezultatov
Quantum-cascade laser-based vibrational circular dichroism.
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Prijava / prijava
Vibrational circular dichroism (VCD) spectra were recorded with a tunable external-cavity quantum-cascade laser (QCL). In comparison with standard thermal light sources in the IR, QCLs provide orders of magnitude more power and are therefore promising for VCD studies in strongly absorbing solvents.
Femtosecond laser-patterned nanopore arrays for surface-mediated peptide treatment.
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The major goal of this study was to create easy-to-use, reusable substrates capable of storing any peptides or bioactive molecules for a desired period of time until cells uptake them without the need for bioactive molecule or peptide-specific techniques. Nanopore arrays of uniform size and
Differential effects of Nd-YAG laser on collagen and elastin production by chick embryo aortae in vitro. Relevance to laser angioplasty for removal of atherosclerotic plaques.
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Aortae from 17-day old chick embryos were subjected to irradiation with a Nd:YAG laser at energy densities varying from 1.2 - 4.7 X 10(3) J/cm2. The aortae were pulse-labeled in vitro with [3H]proline or [14C]valine, and the synthesis of collagenous polypeptides and soluble elastin was examined by
[Effect of laser irradiation on collagen production by fibroblasts in vitro].
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The effects of the low power laser irradiation (Space Mix 5 Mid Laser) on the 3H-proline incorporation in the collagenic proteins produced by two lines of normal human fibroblasts in vitro were studied. The 3H-thymidine incorporation in cultures of control and irradiated fibroblasts of the same
Laser-Raman and infrared spectra of amino acids and their metal complexes. 3. Proline and bisprolinato complexes.
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pH titration monitored by quantum cascade laser-based vibrational circular dichroism.
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Vibrational circular dichroism (VCD) spectra of aqueous solutions of proline were recorded in the course of titrations from basic to acidic pH using a spectrometer equipped with a quantum cascade laser (QCL) as an infrared light source in the spectral range from 1320 to 1220 cm(-1). The pH-dependent
Muscilage characterization, biochemical and enzymatic activities of laser irradiated Lagenaria siceraria seedlings.
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Laser stimulation effect on L. siceraria seed mucilage, biochemicals and enzymatic activities during early growth stages were investigated. The laser density power of 1mW/cm2 for 3 and 5min treatments were performed and various responses i.e., seedlings mucilage, biochemical and enzymatic activities
Influences of proline and cysteine residues on fragment yield in matrix-assisted laser desorption/ionization in-source decay mass spectrometry.
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Matrix-assisted laser desorption/ionization in-source decay produces highly informative fragments for the sequencing of peptides/proteins. Among amino acids, cysteine and proline residues were found to specifically influence the fragment yield. As they are both frequently found in small peptide
EPR and laser flash photolysis studies of the reaction of nitric oxide with water soluble NO trap Fe(II)-proline-dithiocarbamate complex.
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Diethyl-dithiocarbamate (DETC) chelating ferrous iron is used during EPR detection of nitric oxide (.NO) as a precursor of spin traps for the quantification of released .NO both in vitro and in vivo. This method is based on the high affinity of water insoluble iron-dithiocarbamate complexes towards
Stimulation of collagen formation in the intestinal anastomosis by low dose He-Ne laser.
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The effect of low dose He-Ne laser on the healing of intestinal anastomosis was studied in the albino rat. A small piece of jejunum was removed from each rat and the ends sutured back with a simple interrupted pattern. In the experimental animal, the anastomosis was irradiated through an optic fiber
Nonthermal effects of ND:YAG laser on biological functions of human skin fibroblasts in culture.
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Previous studies have indicated that laser can selectively affect the biological functions of cells. In the present study, the role of a thermal component in laser-induced alterations in the biology of human skin fibroblasts was examined. Cells were cultured on 96-well tissue culture plates,
Laser-induced fluorescence detection of 9-fluorenylmethyl chloroformate derivatized amino acids in capillary electrophoresis.
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Laser-induced fluorescence (LIF) was applied to the detection of 9-fluorenylmethyl chloroformate (FMOC-Cl) derivatized amino acids separated by capillary electrophoresis. Fluorescence excitation was provided by a pulsed, KrF laser operating at 248 nm. A limit of detection of 5 x 10(-10) M was
Failure of a helium-neon laser to affect components of wound healing in vitro.
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The red light of a helium-neon (He-Ne) laser has been reported to stimulate wound healing and cell growth. To investigate the nature of its influence on wound healing we have studied seven components of the healing process in vitro: human skin fibroblast, epithelial and endothelial cell
Ultrastructural and autoradiographical analysis show a faster skin repair in He-Ne laser-treated wounds.
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There are evidences that low-intensity red laser radiation is capable to accelerate wound healing. Nowadays, this therapy has been gradually introduced in clinical practice although mechanisms underlying laser effects are poorly understood. To better understand the photobiological effects of laser
Analysis of estrogen-regulated genes in mouse uterus using cDNA microarray and laser capture microdissection.
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The steroid hormone, estrogen, plays an important role in various physiological events which are mediated via its nuclear estrogen receptors, ERalpha and ERbeta. However, the molecular mechanisms that are regulated by estrogen in the uterus remain largely unknown. To identify genes that are
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