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Stran 1 iz 38 rezultatov
Optimization of alkaline protease production by Aspergillus clavatus ES1 in Mirabilis jalapa tuber powder using statistical experimental design.
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Prijava / prijava
Medium composition and culture conditions for the bleaching stable alkaline protease production by Aspergillus clavatus ES1 were optimized. Two statistical methods were used. Plackett-Burman design was applied to find the key ingredients and conditions for the best yield. Response surface
Fibrinolytic serine protease isolation from Bacillus amyloliquefaciens An6 grown on Mirabilis jalapa tuber powders.
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Prijava / prijava
In this study, Mirabilis jalapa tuber powder (MJTP) was used as a new complex organic substrate for the growth and production of fibrinolytic enzymes by a newly isolated Bacillus amyloliquefaciens An6. Maximum protease activity (1,057 U/ml) with casein as a substrate was obtained when the strain was
Proteus mirabilis strains of diverse type have IgA protease activity.
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Prijava / prijava
A strain of Proteus mirabilis associated with chronic urinary tract infection was found to produce an EDTA-sensitive IgA protease that cleaved the IgA heavy chain into two fragments at sites different from those attacked by other microbial IgA1 proteases. Another 14 P. mirabilis strains of diverse
Influence of RP4 plasmid on extracellular protease secretion by Proteus mirabilis.
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Prijava / prijava
The transference by conjugation of protease genetic information between Proteus mirabilis strains only occurs upon mobilization by a conjugative plasmid such as RP4 (Inc P group). Upon receiving the RP4 plasmid, the level of proteolytic activity of the protease-excreting P. mirabilis is reduced to
The production and activity in vivo of Proteus mirabilis IgA protease in infections of the urinary tract.
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Prijava / prijava
Immunoblotting of urine from 21 patients of both sexes and of wide age range who had a Proteus mirabilis urinary tract infection (UTI) showed that 14 (64%) specimens contained immunoglobulin A (IgA). In nine (64%) of these the IgA heavy chain had been degraded to fragments of a size identical to
Identification of protease and rpoN-associated genes of uropathogenic Proteus mirabilis by negative selection in a mouse model of ascending urinary tract infection.
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Prijava / prijava
Proteus mirabilis, a motile gram-negative bacterium, is a principal cause of urinary tract infections in patients with functional or anatomical abnormalities of the urinary tract or those with urinary catheters in place. Thus far, virulence factors including urease, flagella, haemolysin, various
The Lon protease regulates swarming motility and virulence gene expression in Proteus mirabilis.
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A mini-Tn5lacZ1 transposon insertion in a gene encoding an orthologue of the Lon protease conferred a hyper-swarming phenotype on Proteus mirabilis. The lon mutation increased the accumulation of mRNA for representative class 1 (flhDC), class 2 (fliA) and class 3 (flaA) genes during swarmer cell
ZapA, a possible virulence factor from Proteus mirabilis exhibits broad protease substrate specificity.
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Prijava / prijava
The opportunistic bacterium Proteus mirabilis secretes a metalloprotease, ZapA, considered to be one of its virulence factors due to its IgA-degrading activity. However, the substrate specificity of this enzyme has not yet been fully characterized. In the present study we used fluorescent peptides
OBSERVATIONS ON A PROTEASE FROM PROTEUS MIRABILIS.
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Structural characterization of Spinacia oleracea trypsin inhibitor III (SOTI-III).
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Prijava / prijava
In recent decades, several canonical serine protease inhibitor families have been classified and characterized. In contrast to most trypsin inhibitors, those from garden four o'clock (Mirabilis jalapa) and spinach (Spinacia oleracea) do not share sequence similarity and have been proposed to form
Effector specialization in a lineage of the Irish potato famine pathogen.
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Accelerated gene evolution is a hallmark of pathogen adaptation following a host jump. Here, we describe the biochemical basis of adaptation and specialization of a plant pathogen effector after its colonization of a new host. Orthologous protease inhibitor effectors from the Irish potato famine
Genes associated with opening and senescence of Mirabilis jalapa flowers.
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Prijava / prijava
A modest ethylene climacteric accompanies flower senescence in Mirabilis jalapa L., and exogenous ethylene accelerates the process. However, inhibitors of ethylene action and synthesis have little effect on the life-span of these ephemeral flowers. Treatment with alpha-amanitin, an inhibitor of
Co-ordinate expression of virulence genes during swarm-cell differentiation and population migration of Proteus mirabilis.
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Prijava / prijava
The uropathogenic Gram-negative bacterium Proteus mirabilis exhibits a form of multicellular behaviour termed swarming, which involves cyclical differentiation of typical vegetative cells into filamentous, multinucleate, hyperflagellate swarm cells capable of rapid and co-ordinated population
Molecular analysis of a metalloprotease from Proteus mirabilis.
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Prijava / prijava
Proteus mirabilis is known for its ability to differentiate from swimmer to swarmer cells, a process crucial for the pathogenesis of these bacteria during urinary tract infections. Among the many virulence factors produced during swarmer cell differentiation is an extracellular metalloprotease. A
[The secreted proteolytic enzymes of Proteus mirabilis].
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Prijava / prijava
Experiments on white mice, made with the use of genetically linked pair of P. mirabilis differing in the presence of protease activity, have demonstrated the role of this activity in the aggravation of the infectious process, observed only in cases of the parenteral introduction of microorganisms.
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