oleanolic acid glycoside/vrtni ognjič

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Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

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Prijava / prijava

In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode

Distribution of oleanolic acid glycosides in vacuoles and cell walls isolated from protoplasts and cells of Calendula officinalis leaves.

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Prijava / prijava

The contents of oleanolic acid and its 3-0-glucuronide derivatives as well as of 3-0-glucoside derivatives were determined in vacuoles prepared from protoplasts and cell walls obtained from cells of Calendula officinalis leaves. In both cell compartments studied 37% of total cellular oleanolic acid

Metabolism of a new series of oleanolic acid glycosides in Calendula officinalis shoots.

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Competition between oleanolic acid glycosides in their transport to isolated vacuoles from Calendula officinalis leaf protoplasts.

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[On distribution of oleanolic acid glycoside and on bitter principle in the marigold Calendula officinalis L].

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Specificity of the tonoplast transport of the oleanolic acid monoglycosides in the vacuoles from Calendula officinalis leaves.

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The specificity of two separate tonoplast permeases transporting oleanolic acid glycosides was investigated in vacuoles isolated from leaf protoplasts of marigold (Calendula officinalis) with the use of chemically synthesized analogs. The results indicate that the proper structure of both parts of

Increased synthesis of a new oleanane-type saponin in hairy roots of marigold (Calendula officinalis) after treatment with jasmonic acid.

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Prijava / prijava

Native plant of marigold (Calendula officinalis L.) synthesizes oleanolic acid saponins classified as glucosides or glucuronides according to the first residue in sugar chain bound to C-3 hydroxyl group. Hairy root culture, obtained by transformation with Agrobacterium rhizogenes strain 15834,

Metabolism of [3-3H]oleanolic acid in the isolated Calendula officinalis leaf cells and transport of the synthesized glycosides to the cell wall and the extracellular space.

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It has been shown for the first time that [3-3H]oleanolic acid glycosides formed in the cytosol of C. officinalis leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-3H]oleanolic acid 3-O-monoglucuronide (29%) as well as

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