pyruvate kinase/tobak

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Altered growth of transgenic tobacco lacking leaf cytosolic pyruvate kinase.

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Prijava / prijava

Previously, we reported that transformation of tobacco (Nicotiana tabacum L.) with a vector containing a potato cytosolic pyruvate kinase (PKc) cDNA generated two plant lines specifically lacking leaf PKc (PKc-) as a result of co-suppression. PKc deficiency in these primary transformants did not

Normal growth of transgenic tobacco plants in the absence of cytosolic pyruvate kinase.

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Prijava / prijava

The coding sequence of the cytosolic isozyme of potato tuber pyruvate kinase (PK) was attached to the transit peptide of the small subunit of pea ribulose-1,5-bisphosphate carboxylase oxygenase and placed under the control of the cauliflower mosaic virus 35S promoter. This construct was transformed

Molecular characterization of plastid pyruvate kinase from castor and tobacco.

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Prijava / prijava

Clones encoding two different forms of plastid pyruvate kinase (PKp; EC 2.7.1.40) have been isolated from both castor and tobacco seed cDNA libraries. One form, designated PKpA, from castor was described in a previous report, and the tobacco homologue of PKpA has now been isolated. In addition, a

Photosynthesis and carbon partitioning in transgenic tobacco plants deficient in leaf cytosolic pyruvate kinase

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Prijava / prijava

Whole-plant diurnal C exchange analysis provided a noninvasive estimation of daily net C gain in transgenic tobacco (Nicotiana tabacum L.) plants deficient in leaf cytosolic pyruvate kinase (PKc-). PKc- plants cultivated under a low light intensity (100 &mgr;mol m-2 s-1) were previously shown to

In planta proximity-dependent biotin identification (BioID) identifies a TMV replication co-chaperone NbSGT1 in the vicinity of 126 kDa replicase.

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Prijava / prijava

Tobacco mosaic virus (TMV) is a positive, single-stranded RNA virus. It encodes two replicases (126 kDa and 183 kDa), a movement protein and a coat protein. These proteins interact with host proteins for successful infection. Some host proteins such as eEF1α, Tm-1, TOM1, 14-3-3 proteins directly

Antisense inhibition of enolase strongly limits the metabolism of aromatic amino acids, but has only minor effects on respiration in leaves of transgenic tobacco plants.

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Prijava / prijava

Enolase catalyses the reversible conversion of 2-phosphoglycerate and phosphoenolpyruvate in glycolysis. Phosphoenolpyruvate constitutes an important branch point in plant metabolism. It is converted to pyruvate by pyruvate kinase and organic acids by phosphoenolpyruvate carboxylase.

Rapid Metabolic Changes in the Wounding Response of Leaf Discs following Excision.

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Prijava / prijava

The dark respiration rate of discs from fully expanded tobacco leaves (Nicotiana tabacum) increased linearly with decreasing diameter, the relative increase being independent of leaf age. The wound respiration responsible for this situation reached a plateau within 15 minutes of excision. Metabolite

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