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13 rezultatov
Presence of trifoliin A, a Rhizobium-binding lectin, in clover root exudate.
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Prijava / prijava
Trifoliin A, a Rhizobium-binding glycoprotein from white clover, was detected in sterile clover root exudate by a sensitive immunofluorescence assay employing encapsulated cells of Rhizobium trifolii 0403 heat-fixed to microscope slides. Its presence in root exudate was further examined by
Development and trifoliin A-binding ability of the capsule of Rhizobium trifolii.
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Prijava / prijava
The age-dependent lectin-binding ability of Rhizobium trifolii 0403 capsular polysaccharide (CPS) was examined by following the development of the capsule and its ability to interact with the white clover lectin trifoliin A. Bacteria grown on agar plates for 3, 5, 7, 14, and 21 days were examined by
Alteration of the Trifoliin A-Binding Capsule of Rhizobium trifolii 0403 by Enzymes Released from Clover Roots.
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Prijava / prijava
The effect of white clover root exudate on capsules of Rhizobium trifolii 0403 was examined. The clover lectin trifoliin A was detected in root exudate of two clover varieties by indirect immunofluorescence with antibody against this lectin purified from clover seed. Trifoliin A bound uniformly to
Growth-phase-dependent immunodeterminants of Rhizobium trifolii lipopolysaccharide which bind trifoliin A, a white clover lectin.
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Prijava / prijava
The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to
Trifoliin A: a Rhizobium-recognition lectin in white clover roots.
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Prijava / prijava
Effect of nitrate supply on the in-vivo synthesis and distribution of trifollin A, a Rhizobium trifolii-binding lectin, in Trifolium repens seedlings.
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Prijava / prijava
In-vivo synthesis of the white-clover lectin, trifoliin A, was examined by the incorporation of labeled amino acids into protein during heterotrophic growth of intact Trifolium repens L. seedlings. Lectin synthesis was quantified by measuring the level of labeled protein immunoprecipitated from root
The effect of interspecies transfer of Rhizobium host-specific nodulation genes on acidic polysaccharide structure and in situ binding by host lectin.
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Prijava / prijava
Host specificity in the Rhizobium-legume symbiosis is controlled in the bacterium by host specific nodulation (hsn) genes residing on its symbiotic plasmid. We have examined the structure of the major acidic heteropolysaccharide produced by recombinant hybrid strains of Rhizobium leguminosarum
Characterization of the anomalous infection and nodulation of subterranean clover roots by Rhizobium leguminosarum 1020.
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Prijava / prijava
Anomalous nodulation of Trifolium subterraneum (subterranean clover) roots by Rhizobium leguminosarum 1020 was examined as a model of modified host-specificity in a Rhizobium-legume symbiosis. Consistent with previous reports, these nodules (i) appeared most often at sites of secondary root
Stimulation of clover root hair infection by lectin-binding oligosaccharides from the capsular and extracellular polysaccharides of Rhizobium trifolii.
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Prijava / prijava
A polysaccharide depolymerase isolated from the phage lysate of Rhizobium trifolii 4S was used to fragment capsular polysaccharides (CPS) and extracellular polysaccharides (EPS) of R. trifolii 0403 into oligosaccharides. These products were analyzed for clover lectin (trifoliin A)-binding ability,
Trifolin: a Rhizobium recognition protein from white clover.
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Prijava / prijava
A protein agglutinin, trifoliin, was purified from white clover seeds and seedling roots. Trifoliin specifically agglutinates the symbiont of clover, Rhizobium trifolii, at concentrations as low as 0.2 microgram protein/ml, and binds to the surface of encapsulated R. trifolii 0403. This clover
Regulation by fixed nitrogen of host-symbiont recognition in the Rhizobium-clover symbiosis.
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Prijava / prijava
Either NO(3) (-) (16 millimolar) or NH(4) (+) (1 millimolar) completely inhibited infection and nodulation of white clover seedlings (Trifoliin repens) inoculated with Rhizobium trifolii. The binding of R. trifolii to root hairs and the immunologically detectable levels of the plant lectin,
Alteration of surface properties in a Tn5 mutant strain of Rhizobium trifolii 0403.
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Prijava / prijava
A symbiotically defective mutant strain of Rhizobium trifolii, UR251, was obtained by transposon Tn5 mutagenesis of R. trifolii 0403 rif and recognized by its partially ineffective (Fix +/-) phenotype on white clover plants. UR251 had a single Tn5 insertion in plasmid DNA, a wild-type plasmid
Specific phases of root hair attachment in the Rhizobium trifolii-clover symbiosis.
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Prijava / prijava
The time course and orientation of attachment of Rhizobium trifolii 0403 to white clover root hairs was examined in slide cultures by light and electron microscopy. Inocula were grown for 5 days on defined BIII agar medium and represented the large subpopulation of fully encapsulated single cells
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