Environmental Enterovirus Monitoring
Fjalë kyçe
Abstrakt
Përshkrim
Environmental Monitoring Program for Enterovirus (1) Case Collection. The studies cases include patients who were suspected of having enterovirus illnesses, such as HFMD and herpangina. Institutional review board approval was obtained from the Hospital for this study and informed consent was obtained from all patients or their parents. (2) Questionnaire. questionnaire based interviews were used to collect information about the family members, including demographic data, the number of bedrooms in the house, amount of contact time with the patient, presence and pattern of current or recent signs and symptoms. (3) Patient and culture samples. Laboratory evidence of enterovirus infection was defined as the isolation of enterovirus from a throat , and rectal swab sample. (4) Environmental Sampling. (a) Air sampling. For the filter/real-time qPCR assay, the air in each ward was filtered through a 37-mm-diameter Nuclepore filter (Costar, Cambridge, MA), which is a track-etched polycarbonate filter consisting of a polycarbonate membrane with straight-through pores of uniform size (0.4 μm). The filters were supported by cellulose pads and loaded into open-face three-piece plastic cassettes. The pump and filter apparatus were placed within 1 m from the patient's bed on an adjacent nightstand. (b) Surface sampling Regarding surface sampling, surfaces of environment and equipment were sampled with moistened sterile cotton swabs.
Datat
Verifikuar së fundmi: | 03/31/2005 |
Paraqitur së pari: | 09/11/2005 |
Regjistrimi i vlerësuar u dorëzua: | 09/11/2005 |
Postuar së pari: | 09/14/2005 |
Përditësimi i fundit i paraqitur: | 09/11/2005 |
Përditësimi i fundit i postuar: | 09/14/2005 |
Data e fillimit të studimit aktual: | 04/30/2005 |
Data e vlerësimit të përfundimit të studimit: | 05/31/2005 |
Gjendja ose sëmundja
Faza
Kriteret e pranimit
Gjinitë e pranueshme për studim | All |
Pranon Vullnetarë të Shëndetshëm | po |
Kriteret | Inclusion Criteria: - any case of enterovirus infection Exclusion Criteria: - not enterovirus infection cases |