Sildenafil Activates Browning of White Adipose and Improves Insulin Sensitivity
Fjalë kyçe
Abstrakt
Përshkrim
The study subjects will be taken placebo (first stage) and sildenafil (second stage) intervention for 7 days, respectively afterward.Subcutaneous fat tissue and muscle samples will be obtained by biopsy from some individuals and measure the browning of white adipose tissue and insulin signaling.The insulin sensitivity will be tested by insulin clamp assay before and after each intervention, respectively. In addition,blood samples for biochemical analysis will be obtained before and after each intervention, respectively.
The browning of white adipose tissue will be measured by the expressions of peroxisome proliferator-activated receptor-γ (PPARγ), PPARγcoactivator 1α (PGC-1α), uncoupling protein 1 (UCP-1), the second messenger cyclic guanosine-3', 5'-monophosphate (cGMP),PR domain containing 16 zinc finger transcription factor (Prdm16) and deiodinase, iodothyronine, type II (DIO2).The metabolic makers will be measured by blood pressure, heart rate, thyroid functions, resting metabolic rate, respiratory quotient, blood cGMP, blood insulin and blood glucose.
Datat
Verifikuar së fundmi: | 07/31/2016 |
Paraqitur së pari: | 08/01/2015 |
Regjistrimi i vlerësuar u dorëzua: | 08/10/2015 |
Postuar së pari: | 08/13/2015 |
Përditësimi i fundit i paraqitur: | 08/30/2016 |
Përditësimi i fundit i postuar: | 09/01/2016 |
Data e fillimit të studimit aktual: | 07/31/2015 |
Data e vlerësuar e përfundimit primar: | 08/31/2016 |
Data e vlerësimit të përfundimit të studimit: | 08/31/2016 |
Gjendja ose sëmundja
Ndërhyrja / trajtimi
Drug: Sildenafil
Drug: Placebo group
Faza
Grupet e krahëve
Krah | Ndërhyrja / trajtimi |
---|---|
Experimental: Sildenafil Eleven patients receive sildenafil 100mg/day (25 mg at 8 AM plus 25 mg at 4 PM plus 50 mg at 10 PM)for 7 days. | Drug: Sildenafil sildenafil 100 mg per day for 7 days. |
Placebo Comparator: Placebo group An identical placebo for 7 days in placebo group. | Drug: Placebo group an identical placebo per day for 7 days |
Kriteret e pranimit
Moshat e pranueshme për studim | 20 Years Për të 20 Years |
Gjinitë e pranueshme për studim | Male |
Pranon Vullnetarë të Shëndetshëm | po |
Kriteret | Inclusion Criteria: - overweight volunteers - 20-30 years old - body mass index >=25 kg/m2 - normal glucose tolerance Exclusion Criteria: - normal body mass index - abnormal cardiovascular status - women - history of any local or systemic infectious disease with fever or requiring antibiotic within four weeks of drug administration - current addiction to alcohol or substances of abuse - children - current addiction to alcohol or substances of abuse - mental incapacity - the use of any medication within four weeks - subjects with hyperthyroidism or hypothyroidism, hypertension (even if controlled with medications) |
Rezultati
Masat Kryesore të Rezultateve
1. Browning of white adipose tissue [7 days]
Masat dytësore të rezultateve
1. Improvement of insulin sensitivity [7days]
Masat e tjera të rezultateve
1. Change of metabolic status [3 months]