TRPV Expression in Subjects With Sensitive Skin
Fjalë kyçe
Abstrakt
Përshkrim
Sensitive skin syndrome is defined as the presence of burning, itching or any other unpleasant sensation on the skin, due to physical, chemical or psychological factors. It is frequently a self-diagnosed condition, and there are no accurate tests to recognize or quantify it because of the individual variations in perception and intensity of the related symptoms.
Although the pathogenesis of sensitive skin syndrome is not completely understood, the most accepted theory is the presence of an altered barrier function. Irritation results from the abnormal penetration of substances to deeper layers of the skin, where they can induce vasodilation and stimulate c-type neuronal fibers. Also, changes in the pH of the stratum corneum have been found to induce skin sensitivity through the activation of the transient potential receptor vanilloid (TRPV) neuronal receptors.
TRPV1 was first discovered in 1997, when it was identified as the specific receptor for capsaicin in a subgroup of nociceptors. It is a non-selective thermo-sensitive cationic channel that can be found in nerves from the central and peripheral nervous system, fibroblasts, smooth muscle, mast cells, endothelial cells, gastrointestinal, respiratory and urinary epithelial cells. TRPV1 can be activated by excessive heat (>42ºC), acidic pH (< 5.9), and also by endogenous substances such as N- arachidonoyl dopamine, leucotriene B, phospholipase C, and many others.
In 2001, the functional expression of TRPV1 was identified in human keratinocytes. Their physiologic role in the skin has not been completely understood, but they have been related to differentiation, proliferation, inflammation and homeostasis of the epidermal barrier. Their presence in keratinocytes and cutaneous nervous fibers suggests a role in the sensitive function of the epidermis. It has been proved that the stimulation of TRPV1 in neuronal cells can induce pruritus and burning sensation. In vitro studies have demonstrated that the exogenous stimulation of TRPV1 in keratinocytes induces the release of nitric oxide, ATP, dopamine, prostaglandins, and other pro-inflammatory substances that can act as paracrine mediators between keratinocytes and cutaneous nerve fibers. Therefore, there are scientific bases to hypothesize that an increase in the expression of these receptors can be the responsible for the sensitive skin syndrome.
Datat
Verifikuar së fundmi: | 10/31/2015 |
Paraqitur së pari: | 05/26/2013 |
Regjistrimi i vlerësuar u dorëzua: | 06/05/2013 |
Postuar së pari: | 06/06/2013 |
Përditësimi i fundit i paraqitur: | 11/29/2015 |
Përditësimi i fundit i postuar: | 12/01/2015 |
Data e fillimit të studimit aktual: | 04/30/2013 |
Data e vlerësuar e përfundimit primar: | 08/31/2016 |
Data e vlerësimit të përfundimit të studimit: | 08/31/2016 |
Gjendja ose sëmundja
Ndërhyrja / trajtimi
Procedure: Skin biopsy
Procedure: Oral mucosa specimen
Faza
Grupet e krahëve
Krah | Ndërhyrja / trajtimi |
---|---|
Experimental: Sensitive Skin Subjects with sensitive skin, diagnosed by the lactic acid stinging test. Skin biopsy Oral mucosa specimen | |
Active Comparator: Non-sensitive skin Subjects without sensitive skin, determined by a negative lactic acid stinging test Skin biopsy Oral mucosa specimen |
Kriteret e pranimit
Moshat e pranueshme për studim | 18 Years Për të 18 Years |
Gjinitë e pranueshme për studim | All |
Pranon Vullnetarë të Shëndetshëm | po |
Kriteret | Inclusion Criteria: - Age > 18 years old - Known response to the lactic acid stinging test - Informed signed consent Exclusion Criteria: - Any dermatoses in the test area - Use of topical medications in the test area - Personal history of keloid or hypertrophic scarring - Known allergy to lidocaine - Know heart disease - Pregnancy - Breastfeeding |
Rezultati
Masat Kryesore të Rezultateve
1. Expression of TRPV1 [Up to 1 year]
Masat dytësore të rezultateve
1. TRPV1 and Sensitive Skin [Up to 1 year]
2. TRPV1 and skin phototype [Up to 1 year]
3. TRPV1 and barrier function [Up to 1 year]
Masat e tjera të rezultateve
1. TRPV1 mRNA in biopsies [Up to 1 year]
2. TRPV1 in biopsies by immunohistochemistry [Up to 1 year]
3. mRNA1 of TRPV1 in oral keratinocytes [Up to 1 year]
4. TRPV1 in oral keratinocytes by immunohistochemistry [Up to 1 year]