Vascular Effects of Triglyceride-rich Lipoproteins

Vetëm përdoruesit e regjistruar mund të përkthejnë artikuj

Identifikohuni Regjistrohu

Lidhja ruhet në kujtesën e fragmenteve

StatusiPërfunduar

Sponsorët

King's College London

Bashkëpunëtorë

Royal Veterinary College

TRAJTIM KLINIKAL: NCT01618071

BioSeek: nct01618071

Fjalë kyçe

Abstrakt

Many types of cardiovascular disease begin when the layer of cells lining blood vessels (endothelial cells) start to function abnormally. This causes white blood cells (monocytes) to enter the blood vessel wall and eventually form lesions. Fats from foods we consume are carried in the blood for 3-8 hours after a fatty meal in small particles known as chylomicrons (CM) and chylomicron remnants (CMR). The overall aim of this project is to investigate the idea that n-3 polyunsaturated fatty acids (PUFA) protect against heart disease by modifying the effect of CMR on endothelial cells and monocytes. We hypothesize that n3-PUFA carried in CMR reduce detrimental events which promote blood vessel damage and activate protective mechanisms to improve the function of arteries.

Datat

Verifikuar së fundmi:	08/31/2019
Paraqitur së pari:	06/07/2012
Regjistrimi i vlerësuar u dorëzua:	06/10/2012
Postuar së pari:	06/12/2012
Përditësimi i fundit i paraqitur:	09/11/2019
Përditësimi i fundit i postuar:	09/15/2019
Data e fillimit të studimit aktual:	05/31/2012
Data e vlerësuar e përfundimit primar:	09/30/2012
Data e vlerësimit të përfundimit të studimit:	09/30/2012

Gjendja ose sëmundja

Postprandial Period

Lipemia

Vasodilation

Vascular Resistance

Nitric Oxide

Ndërhyrja / trajtimi

Dietary Supplement: High-fat meals varying in their fatty acid composition

Faza

Grupet e krahëve

Krah	Ndërhyrja / trajtimi
Active Comparator: Oleic acid 75 g high oleic acid sunflower oil.
Active Comparator: Linoleic acid 75 g high linoleic acid sunflower oil.
Experimental: Eicosapentaenoic acid and docosahexaenoic acid 5 g EPA and DHA derived from fish oil, made up to a total of 75 g with high oleic sunflower oil.
Experimental: Docosahexaenoic acid 5 g DHA derived from algal oil, made up to a total of 75 g with high oleic sunflower oil.

Kriteret e pranimit

Moshat e pranueshme për studim	35 Years Për të 35 Years
Gjinitë e pranueshme për studim	Male
Pranon Vullnetarë të Shëndetshëm	po
Kriteret	Inclusion Criteria: - Healthy males - Non-smokers - Aged 35-70 years - Fasting TAG concentrations ≥1.2 mmol/L. Exclusion Criteria: - Reported history of CVD (myocardial infarction, angina, venous thrombosis, stroke), impaired fasting glucose/uncontrolled type 2 diabetes (or fasting glucose ≥ 6.1 mmol/L), cancer, kidney, liver or bowel disease. - Presence of gastrointestinal disorder or use of drug, which is likely to alter gastrointestinal motility or nutrient absorption. - History of substance abuse or alcoholism (previous weekly alcohol intake >60 units/men) - Current self-reported weekly alcohol intake exceeding 28 units - Allergy or intolerance to any component of test meals - Unwilling to restrict consumption of any source of fish oil for the length of the study - Weight change of >3kg in preceding 2 months - Body Mass Index <20 and >35 kg/m2 - Fasting blood cholesterol > 7.8 mmol/L - Current cigarette smoker. - Current use of lipid lowering medication

Rezultati

Masat Kryesore të Rezultateve

1. Activation of inflammatory/oxidative stress pathways within cultured endothelial cells following treatment with 6 h postprandial chylomicron remnant-rich lipoprotein fraction [6 h post-meal]

The primary outcome of the study is activation of inflammatory/oxidative stress pathways within cultured endothelial cells following incubation with pooled postprandial lipoprotein fractions rich in chylomicron remnants. Due to the nature of this type of research this necessitates more than one primary outcome measure: the primary measures are NF-kappa-beta activation, cytokine production (e.g. interleukin-6) and reactive oxygen species generation in the cultured human endothelial cells.

Masat dytësore të rezultateve

1. Incremental area under the plasma concentration versus time curve (iAUC) of triacylglycerol [0, 1, 2, 3, 4, 5 and 6 h post-meal]

2. Incremental area under the plasma concentration versus time curve (iAUC) of glucose [0, 1, 2, 3, 4, 5 and 6 h post-meal]

3. Incremental area under the plasma concentration versus time curve (iAUC) for non-esterified fatty acids [0, 1, 2, 3, 4, 5 and 6 h post-meal]

4. Incremental area under the plasma concentration versus time curve (iAUC) for plasma fatty acid composition (%) [0, 1, 2, 3, 4, 5 and 6 h post-meal]

5. Incremental area under the plasma concentration versus time curve (iAUC) for cholesterol [0, 1, 2, 3, 4, 5 and 6 h post-meal]

6. Incremental area under the unit measure versus time curve for brachial augmentation index [0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 min post-meal]

7. Incremental area under the unit measure versus time curve for systolic blood pressure [0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 min post-meal]

8. Incremental area under the unit measure versus time curve for diastolic blood pressure [0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 min post-meal]

9. Change in digital volume pulse stiffness index [0, 2, 4 and 6 h post-meal]

10. Change in digital volume pulse reflection index [0, 2, 4 and 6 h post-meal]

11. Change in plasma nitrite/nitrate concentrations [0, 2, 4 and 6 h]

12. Change in plasma 8-isoprostane F2alpha concentrations [0, 2, 4 and 6 h post-meal]

13. Activation of inflammatory/oxidative stress pathways within cultured endothelial cells following treatment with 4 h postprandial chylomicron remnant-rich lipoprotein fraction [4 h post-meal]

14. Activation of inflammatory/oxidative stress pathways within cultured endothelial cells following treatment with 5 h postprandial chylomicron remnant-rich lipoprotein fraction [5 h post-meal]