ROLE OF ANTI-INFALMMMATORY INTERVENTIONS IN HIGH FAT DIET INDUCED OBESITY.

Lipotoxicity is defined as deposition of excess fat associated with an inflammatory response. Metabolomic analysis of fatty acids can be a marker of silent inflammation. ω3 enriched diet, celecoxib and safranal may have a protective anti-inflammatory role. Total fatty acids were extracted from RBCs and AA/EPA was assessed using gas chromatographic-mass spectral (GCMS) assay by sensitive single ion monitoring. The present study was conducted on 64 male rats divided into 8 groups; I controls, II received High fat diet (HFD), III received ω-6 enriched HFD, IV received ω-3 enriched HFD, V received celecoxib with HFD, VI received safranal with HFD, VII and VIII received celecoxib and safranal with ω-3 HFD received respectively. GCMS was performed for analysis of FAME. ELISA assay for serum IL-6 and TGF-β1 levels was used. Statistically significant decrease of AA/EPA ratio was shown in group VII when compared to the groups receiving HFDs. It also showed lowest serum IL-6 level and highest level of TGF- -β1.Conclusion:ω3 enriched diet and drugs such as celecoxib and herbals such as safranal may have anti-inflammatory effect in lipotoxicty. GC-MS with single ion monitoring is valid for the analysis of fatty acids.