RP-LC determination of oleane derivatives in Terminalia arjuna.

A rapid sensitive and reproductive reversed phase high performance liquid chromatographic method with photo diode arrray detection is described for the simultaneous quantification of major oleane derivatives: arjunic acid (4), arjunolic acid (3), arjungenin (2) and arjunetin (1) in Terminalia arjuna extract. The method involves the use of a Waters Spherisorb S10 ODS2 column (250 x 4.6 mm, I.D., 10 microm) and binary gradient mobile phase profile. The various other aspects of analysis viz. Extraction efficiency, peak purity and similarity were validated using a photo diode array detector.