Ultrasound-assisted extraction of alantolactone and isoalantolactone from Inula helenium roots.

This work deals with ultrasound-assisted extraction (UAE) of alantolactone and isoalantolactone from the roots of Inula helenium L., a well-known medicinal plant. The effects of ethanol concentration, extraction time, temperature and number of extraction steps on the extraction yields of both sesquiterpene lactones were investigated. Gas chromatographic (GC) method was used for simultaneous determination of their contents in the corresponding extracts. A comparison with classical extraction methods [maceration, infusion and micro steam distillation-extraction (MSDE)] showed that the amounts of alantolactone and isoalantolactone achieved by UAE with 70 and 96% EtOH for 30 min at room temperature were higher or almost equal to those obtained by maceration for 24 hours.