pseudorabies/kaliumi

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[Resistance of Aujeszky disease viruses, present in the blood of pigs, to potassium thiosulphate and hydrogen chloride].

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A trial was conducted to study the sensitivity of the Aujeszky disease virus, present in the blood of pigs, to the effect of two preservatives: potassium thiosulphate ( K2S2O5 ) and hydrogen chloride (HCl). The sensitivity of the virus was determined on rabbits infected with pig blood containing

Physicochemical properties of pseudorabies virus hemagglutinin.

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Pseudorabies virus hemagglutinin was readily adsorbed on mouse erythrocytes at 4, 22, or 37 degrees C, but not on cattle erythrocytes. The adsorbed hemagglutinin could not be eluted from the cells by resuspending in phosphate-buffered saline (PBS), by incubating at 37 or 50 degrees C, or by

Cellular gene expression survey of PseudoRabies Virus (PRV) infected Human Embryonic Kidney cells (HEK-293).

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Pseudorabies virus (PRV) is an alpha herpesvirus that causes Aujezsky disease in the pig. To characterize the impact of PRV infection on cellular expression, we used microarrays consisting of 9850 oligonucleotides corresponding to human genes and examined the expression levels of mRNA isolated 0.5,

Stability of pseudorabies virus during freeze-drying and storage: effect of suspending media.

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The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various

Preservation of red cell properties after virucidal phototreatment with dimethylmethylene blue.

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BACKGROUND All published reports have described methods for virus photoinactivation which significantly alter red cell (RBC) properties during storage. In order to improve virucidal activity and reduce damage to RBCs, a series of phenothiazine derivatives were either synthesized or purified and

Potentiation of antiviral activity of acyclovir by polyene macrolide antibiotics.

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The potentiation of the antiviral activity of acyclovir [9-[(2-hydroxyethoxy)methyl]guanine] by polyene macrolide antibiotics has been studied as a function of the macrolide structure. The 12 polyenes chosen for this study represented the major structural groups of these antibiotics and induced in

Immunological characterization of the feline herpesvirus-1 glycoprotein B and analysis of its deduced amino acid sequence.

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Feline herpesvirus 1 (FHV-1) is an important viral pathogen of cats. Like other alphaherpesviruses, FHV-1 contains a HSV-1 glycoprotein B (gB) homolog. In this study, monospecific antisera to HSV-1 gB reacted with three FHV-1 proteins (100, 64, and 58 kDa) present in virion lysates by

Use of a flexible thiopyrylium photosensitizer and competitive inhibitor for pathogen reduction of viruses and bacteria with retention of red cell storage properties.

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BACKGROUND Progress in developing photochemical methods for pathogen reduction of red blood cells (RBCs) has been hampered by hemolysis. A flexible, nucleic acid-intercalating thiopyrylium (TP) dye that is only photochemically active in the bound state and a competitive inhibitor of RBC membrane

Simple and rapid purification of alphaherpesviruses by chromatography on a cation exchange membrane.

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A simple and rapid method is described for the purification of two alphaherpesviruses, pseudorabies virus (PrV) and bovine herpesvirus 1, by chromatography on a cation exchange membrane. Cell culture supernatants were passed over a sulfonic-acid modified filter membrane and virions were eluted with

Ion channels involved in the presynaptic hyperexcitability induced by herpes virus suis in rat superior cervical ganglion.

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Rat superior cervical ganglia infected with herpes virus suis (pseudorabies virus) display a spontaneous bursting activity of still unknown origin. Previous intracellular recordings from the ganglionic neurons combined with pharmacological studies showed that the postganglionic action potentials are

Native De Novo Structural Determinations of Non-Canonical Nucleic Acid Motifs by X-ray Crystallography at Long Wavelengths

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Obtaining phase information remains a formidable challenge for nucleic acid structure determination. The introduction of an X-ray synchrotron beamline designed to be tunable to long wavelengths at Diamond Light Source has opened the possibility to native de novo structure determinations by the use

Additive effects of dipyridamole and Trolox in protecting human red cells during photodynamic treatment.

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OBJECTIVE Photodynamic treatment (PDT) of red blood cell (RBC) suspensions has been reported to result in virus inactivation, but also in deterioration of cell quality. Recently, we have demonstrated the potential usefulness of the reactive oxygen species scavenger dipyridamole in selectively

[Studies on inactivation of viruses in drinking and surface water. A contribution to the decontamination of water by field methods (author's transl)].

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The suitability for field use of heating up to 80 degrees C and adding six different virucidal chemicals for decontamination of drinking and surface water was investigated using the viruses of Polio (vaccine strain), ECBO, Reo, bovine Parvo, HCC, Pseudorabies, ND and Vaccinia. The Parvovirus

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