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Studies on the deterioration of antigen E in extracts of Ambrosia elatior.

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Five per cent (w/v) extracts of defatted Ambrosia elatior pollen were prepared in bicarbonate-buffered saline (Coca's extract) and Coca's extract with 50% glycerol (Gly-Coca's extract). Sterile extracts were stored in untreated glassware and glassware coated with silicone. Stock extracts were

Antigen deterioration rate of aqueous ragweed pollen extract.

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Quantitative immunologic studies with allergens; deterioration of ragweed pollen extracts as measured by precipitation, neutralization, and protein nitrogen analysis; the prevention of deterioration by glycerin.

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Cloning, expression, and characterization of pollen allergens from Humulus scandens (Lour) Merr and Ambrosia artemisiifolia L.

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OBJECTIVE To clone the pollen allergen genes in Humulus scandens (Lour) Merr (LvCao in Chinese) and short ragweed (Ambrosia artemisiifolia L) for recombinant allergen production and immunotherapy. METHODS The allergen genes were selectively amplified in the weed pollen cDNA pool by using a special

More than anti-malarial agents: therapeutic potential of artemisinins in neurodegeneration.

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Artemisinin, also called qinghaosu, is originally derived from the sweet wormwood plant (Artemisia annua), which is used in traditional Chinese medicine. Artemisinin and its derivatives (artemisinins) have been widely used for many years as anti-malarial agents, with few adverse side effects.

Cloning the cDNA encoding the AmbtV allergen from giant ragweed (Ambrosia trifida) pollen.

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Ragweed (Ambrosia) pollens contain a number of proteins that cause allergic disease in ragweed-sensitive people. The cloning of the AmbtV cDNA is important, since the 4.4-kDa AmbtV, one of the allergens in giant ragweed (Ambrosia trifida) pollen, serves as a simple model system to study the basic

[Effects of nasal provocation with a single-dose allergen on the explicit and implicit memory of physically active and inactive patients with ragweed allergy].

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UNASSIGNED Allergy is an endemic disease and has a considerable impact on the quality of life. This study aimed to measure the effect of active allergic rhinitis on memory functions of physically active and inactive patients with ragweed allergy. UNASSIGNED Memory functions were assessed before and

Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles.

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Deep level insect relationships are generally difficult to resolve, especially within taxa of the most diverse and species rich holometabolous orders. In beetles, the major diversity occurs in the Phytophaga, including charismatic groups such as leaf beetles, longhorn beetles and weevils. Bark and

Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration.

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OBJECTIVE To obtain the entire gene open reading frame (ORF) and to construct the expression vectors for recombinant allergen production. METHODS Gene fragments corresponding to the gene specific region and the cDNA ends of pollen allergens of short ragweed (Rg, Ambrosia artemisiifolia L.) were

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