furunculosis/protease

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Differential susceptibility of salmonid fishes to furunculosis correlates with differential serum enhancement of Aeromonas salmonicida extracellular protease activity.

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Rainbow trout, Atlantic salmon and brown trout, respectively, exhibit an increasing order of susceptibility to furunculosis. We investigated if this may be due to a differential sensitivity to exotoxins or ability to inhibit the exoprotease which is required by the bacterium to digest protein for

Significance of Extracellular Protease for Growth of a Heterotrophic Bacterium, Aeromonas salmonicida.

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The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid

In vivo production of A-protein, lipopolysaccharide, iron-regulated outer membrane proteins and 70-kDa serine protease by Aeromonas salmonicida subsp. salmonicida.

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Using specific immunostaining of Western blots, the in vivo expression of several putative virulence factors of Aeromonas salmonicida subsp. salmonicida was demonstrated in infected muscle tissue of Atlantic salmon and rainbow trout. Three virulent isolates of A. salmonicida were used. One isolate

Loss of virulence in a protease-deficient mutant of Aeromonas salmonicida.

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The importance of extracellular protease production by Aeromonas salmonicida, the bacterial pathogen of fish furunculosis, was investigated with four virulent strains (which were autoagglutinative, hemagglutinative, resistant to fish serum, adhesive to fish tissue culture, protease positive,

Hepatic gene expression profiling reveals protective responses in Atlantic salmon vaccinated against furunculosis.

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BACKGROUND Furunculosis, a disease caused with gram negative bacteria Aeromonas salmonicida produces heavy losses in aquaculture. Vaccination against furunculosis reduces mortality of Atlantic salmon but fails to eradicate infection. Factors that determine high individual variation of vaccination

Inhibition of the Aeromonas salmonicida extracellular protease by alpha 2-macroglobulin in the serum of rainbow trout.

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One of the major pathogenic factors of Aeromonas salmonicida, the bacterium causing furunculosis in fish, is considered to be a protease secreted in the extracellular products. This protease can be inhibited by normal trout and rabbit serum but the factor responsible has not hitherto been

The role of alpha 2-macroglobulin in furunculosis: a comparison of rainbow trout and brook trout.

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1. The molecular basis for the high survival rate of rainbow trout, Oncorhynchus mykiss, infected with furunculosis was investigated. 2. Alpha 2-macroglobulin (alpha 2M), a major serum protease inhibitor, was partially purified from rainbow trout and brook trout, Salvelinus fontinalis, sera; the

Thrombotic thrombocytopenic purpura associated with Staphylococcus aureus endocarditis in a diabetic with furunculosis.

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Thrombotic thrombocytopenic purpura (TTP) is an uncommon and potentially fatal syndrome which is due to either congenital or acquired deficiency of the ultra large von Willbrand's factor (ULVWF) cleavage protease. It is characterized by mechanical micro angiopathic hemolytic anemia, and organ

A comparative study of the formation of extracellular proteins by Aeromonas salmonicida at two different temperatures.

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Aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees C, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees C, the optimum temperature for growth. Similar bacterial densities in the

Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida.

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The importance of the two major extracellular enzymes of Aeromonas salmonicida, glycerophospholipid: cholesterol acyltransferase (GCAT) and a serine protease (AspA), to the pathology and mortality of salmonid fish with furunculosis had been indicated in toxicity studies. In this study, the genes

Identification of major common extracellular proteins secreted by Aeromonas salmonicida strains isolated from diseased fish.

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Ten different strains of Aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree C) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. In each case the extracellular proteins that were formed were

Toxicity of Aeromonas salmonicida cells to Atlantic salmon Salmo salar peritoneal macrophages.

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Several strains of Aeromonas salmonicida were toxic to cultured peritoneal macrophages of Atlantic salmon, Salmo salar, in minimum doses of 5 x 10(6) CFU, whereas other strains were not. There was no correlation between cytotoxicity and in vivo virulence of the bacteria, the presence or absence of

Medicinal use of potato-derived products: a systematic review.

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In the traditional medicine of Europe, raw potatoes are used for gastrointestinal disorders, and topical potato preparations as a hot pack for pain or for softening furuncles. The aim of this study was to review the literature and summarize the data on the medicinal use of potato-derived products.

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