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brassica nigra/albumin
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Sinigrin, a major glucosinolate from cruciferous vegetables restrains non-enzymatic glycation of albumin.
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Sinigrin is a major component of cruciferous vegetables such as cabbage, Brussels sprout, mustard greens and broccoli. The present study demonstrates the protective effects of sinigrin against the non-enzymatic glycation of albumin and lens crystallin based on fluorescence spectroscopy, circular
Napins, 2S albumins, are major allergens in oilseed rape and turnip rape.
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BACKGROUND
Children with IgE-mediated allergy to foods frequently react to seeds of oilseed rape (Brassica napus ssp. oleifera) and turnip rape (Brassica rapa ssp. oleifera) in skin prick tests (SPTs). Sensitization pathways are not known.
OBJECTIVE
We identified possible major allergens in oilseed
Behaviour of a protein isolate from rapeseed (Brassica napus) and its main protein components - globulin and albumin - at air/solution and solid interfaces, and in emulsions.
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The behaviour of a rapeseed protein isolate (RI) and its main protein components - globulin (RG) and albumin (RA) - in adsorbed and spread monolayers, as well as in emulsions was investigated. Tensiometry, film-pressure area and Langmuir-Blodgett-techniques, and emulsion parameters were used to
[Isolation and characterization of main albumin fractions of seeds from sunflower (Helianthus annuus L.) and rape (Brassica napus L.)].
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The main fractions of the albumins from sunflower and rapeseeds (isolated by means of precipitation with ammonium sulphate or tannin + caffeine and subsequent gel chromatography) are low-molecular, very basic proteins. Their molecular weights range from 10 000 to 16 000, and their isoelectric points
Expression of the 2S albumin from Bertholletia excelsa in Brassica napus.
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The methionine rich 2S albumin seed storage protein of Bertholletia excelsa has been expressed in seeds of Brassica napus (rapeseed). A chimeric gene driven by the soybean lectin 5' flanking regions was used to produce a fusion protein consisting of the soybean lectin signal peptide and the
Cloning and sequencing of an apparently recombinant promoter for napin gene from Brassica campestris genomic library and its evolutionary significance.
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From a genomic library of Brassica campestris (brown sarson cv. B54), we have cloned and sequenced about 2 kb of upstream regulatory region from one of the 2S albumin-coding gene family. The sequence has several seed-specific promoter motifs. A sequence alignment of the 5' flanking regions of the
Identification, characterization and epitope mapping of proteins encoded by putative allergenic napin genes from Brassica rapa.
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Sensitization to turnip rape and oilseed rape in children with atopic dermatitis: a case-control study.
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Turnip rape and oilseed rape 2S albumins are new allergens in children with atopic dermatitis suspected for food allergy. We recently found that 11% (206/1887) of these children had a positive skin prick test to seeds of oilseed rape (Brassica napus) and/or turnip rape (Brassica rapa). In the
Sensitization and allergy to turnip rape: a comparison between the Finnish and French children with atopic dermatitis.
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OBJECTIVE
Finnish children with atopic dermatitis (AD) are frequently sensitized and show positive food challenge to turnip rape. We examined whether French children are also allergic to this oilseed plant and whether mustard could be the cross-reacting allergen.
METHODS
Turnip rape and mustard
Binding Specificity and Possible Analytical Applications of the Cytokinin-binding Antibody, Anti-N-Benzyladenosine.
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Antibodies elicited in rabbits by immunization with an N(6)-benzyladenosine-bovine serum albumin conjugate bound N(6)-benzyladenosine specifically. The affinity constants and specific binding site concentrations for a number of cytokinins and related compounds were estimated by nonlinear least
Protective effects of broccoli (Brassica oleracea) against oxidative damage in vitro and in vivo.
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The antioxidative effect and protective potential against diabetes of the broccoli flower were investigated both in vitro and in a diabetic rat model. Among fractions of MeOH, CH2Cl2, BuOH, and H2O, the BuOH fraction exerted the strongest inhibitory activities on 1,1-diphenyl-2-picrylhydrazyl
The trypsin-inhibitory, immunostimulatory and antiproliferative activities of a napin-like polypeptide from Chinese cabbage seeds.
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A heterodimeric 13.8 kDa napin-like polypeptide has previously been isolated from Chinese cabbage (Brassica parachinensis) seeds with a procedure involving ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, FPLC-ion exchange chromatography on Mono S and
Deduced amino acid sequence of 2S storage protein from Brassica species and their conserved structural features.
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2S seed storage albumin coding regions from five Brassica species, namely Brassica campestris, B. oleracea, B. nigra, B. juncea, and B. carinata have been cloned by PCR amplification of genomic DNA using oligonucleotide primers and their nucleotide sequences have been determined. These sequences
Chemical and Nutraceutical Studies on Infertility of Albino Rats Induced by Cadmium Chloride
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Background and objective: Infertility in couples is rated one in every eight couple worldwide which affects 15% of couples and a male factor is found to be solely responsible or in conjunction with a female factor in 50% of cases. The
[Effects of different applied nitrogen forms on pakchoi (Brassica chinensis) growth and its carbon and nitrogen accumulation].
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A hydroponic experiment was conducted to study the effects of different applied nitrogen forms, i.e., ammonium, nitrate, glycine, glutamine, alanine, bovine serum albumin (BSA), mixture of glycine and nitrate, and mixture of BSA and nitrate, on the growth and carbon and nitrogen accumulation of
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