gingivitis/protease

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Identification of a cathepsin b-like protease in the crevicular fluid of gingivitis patients.

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Crevicular fluid from gingivitis patients contains significant levels of a cysteine protease which was characterized as the lysosomal protease cathepsin B, as judged by substrate specificity, thiol dependence, pH optimum, kinetic parameters, pH stability, and inhibitor sensitivities. A

Immunization with Porphyromonas gingivalis cysteine protease: effects on experimental gingivitis and ligature-induced periodontitis in Macaca fascicularis.

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Targeting bacterial virulence factors such as proteases for immunization may hold the key to limiting or preventing loss of attachment and alveolar bone in periodontal disease. This study examined the clinical, microbiological, and immununological responses following active immunization with a

Protease activity in gingival crevicular fluid from discrete periodontal sites in humans with periodontitis or gingivitis.

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Sensitive fluorogenic assays were used to compare the protease activities of fluid collected from eight such sites in each of 21 adult patients with gingivitis and 22 with periodontitis. The degradation of N-carbobenzoxy-gly-gly-arginine-AMC, L-arginine-AMC, glyproline-AMC, L-leucine-AMC,

Predicted salivary human protease activity in experimental gingivitis revealed by endoProteo-FASP approach

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Gingivitis is a highly prevalent oral condition that can be studied in humans via the 21-d experimental gingivitis model, which allows for investigations into the induction and resolution of gingivitis. In this study, we used the autolysis of saliva as a source of peptides to predict the activity of

[The correlationship between the protease activity and the epithelial cells in the saliva from the patients with gingivitis or marginal periodontitis (author's transl)].

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Increase of free collagenase and neutral protease activities in the gingival crevice during experimental gingivitis in man.

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Is the chemical prevention of gingivitis necessary to prevent severe periodontitis?

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Gingival inflammation seldom causes discomfort, social embarrassment or loss of function. As most sites with gingival inflammation do not progress to severe periodontal disease, gingivitis should not be considered a public health problem. Periodontitis is always preceded by gingivitis. But most

Effect of erythritol on microbial ecology of in vitro gingivitis biofilms.

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Gingivitis is one of the most common oral infections in humans. While sugar alcohols such as erythritol are suggested to have caries-preventive properties, it may also have beneficial effects in prevention of gingivitis by preventing maturation of oral biofilms. The aim of this study was to assess

Periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid.

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In periodontitis, an effective host-response is primarily related to neutrophils loaded with serine proteases, including elastase (NE) and protease 3 (PR3), the extracellular activity of which is tightly controlled by endogenous inhibitors. In vitro these inhibitors are degraded by gingipains,

Alarm Anti-protease Trappin-2 Negatively Correlates With Proinflammatory Cytokines in Patients With Periodontitis.

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BACKGROUND Trappin-2 is one of the potent biologically active serine protease inhibitor with anti-inflammatory properties that has also been characterized as an "alarm anti-protease". Despite the importance of Trappin-2 in several chronic infections has been demonstrated, its potential involvement

Clinical evaluation of the effect of a proteolytic enzyme mouthwash on plaque and gingivitis in young adults.

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One hundred and thirty-one young adults were used in a controlled study to determine whether a proteolytic mouthwash produced from B. subtilis could help remove existing plaque, decrease the rate of plaque accumulation or reduce the clinical signs of gingivitis. These subjects were stratified by

A new autocatalytic activation mechanism for cysteine proteases revealed by Prevotella intermedia interpain A.

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Prevotella intermedia is a major periodontopathogen contributing to human gingivitis and periodontitis. Such pathogens release proteases as virulence factors that cause deterrence of host defenses and tissue destruction. A new cysteine protease from the cysteine-histidine-dyad class, interpain A,

Acute myocardial infarction elevates serine protease activity in saliva of patients with periodontitis.

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OBJECTIVE There are indications that acute myocardial infarction (AMI) may have an effect on the oral environment, which is reflected in the expression of salivary and gingival proteinases. According to our knowledge, no studies have been carried out to investigate the effect of AMI on the

Protease activity in gingival crevicular fluid: presence of free protease.

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Our aim was to study protease activity in GCF from inflamed sites with or without tissue destruction. 19 patients with both periodontitis and gingivitis sites and 12 patients having gingivitis alone participated in the study. GCF samples were collected by an intracrevicular washing method. The

The short-term effectiveness of non-surgical treatment in reducing protease activity in gingival crevicular fluid from chronic periodontitis patients.

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OBJECTIVE The aim of this study was to evaluate the short-term effect of non-surgical periodontal treatment on protease activity in gingival crevicular fluid (GCF) of patients with chronic periodontitis. METHODS After clinical examination, in which pocket probing depth, probing attachment level,

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