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pectinase/nekros

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Endo-polygalacturonase (PG) was purified from a commercial preparation of Aspergillus niger pectinase by means of carboxymethylcellulose chromatography, preparative isoelectric focusing, and gel permeation through Sephadex G-50. The enzyme was electrophoretically homogeneous and consisted of a
From 2002 to 2004, tomato (Lycopersicon esculentum) plants with external stem lesions, adventitious roots, and necrotic pith that was hollowed or chambered were received by the Clinical Lab of the Plant Protection Department from eight greenhouses in the Riyadh, Abha, and El-Kharj regions of Saudi
Dickeya dadantii is a broad host range phytopathogenic bacterium provoking soft rot disease on many plants including Arabidopsis. We showed that, after D. dadantii infection, the expression of the Arabidopsis BOS1 gene was specifically induced by the production of the bacterial PelB/C pectinases
This study aimed to evaluate the effects of a source of dietary soluble (SF) and insoluble fiber (IF) without or with exogenous carbohydrases (xylanase, β-glucanase, and pectinase) on diarrhea incidence, selected immune responses, and growth performance in enterotoxigenic Escherichia coli

A Polysaccharide Isolated from Codonopsis pilosula with Immunomodulation Effects Both In Vitro and In Vivo.

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In this study, an acidic polysaccharide from Codonopsis pilosula Nannf. var. modesta (Nannf.) L. T. Shen (WCP-I) and its main fragment, WCP-Ia, obtained after pectinase digestion, were structurally elucidated and found to consist of a rhamnogalacturonan I (RG-I) region containing both

[Discovery of phytopathogenic bacteria 100 years ago: transatlantic controversies and polemics].

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The demonstration of a bacterial cause of some plant diseases has been claimed few years after it was commonly recognized that bacteria were able to cause diseases of human and animal. Nevertheless, some sharp controversies took place, between German and American specialists (1897-1901), before the
Phylogenetic relationships among 18 isolates in the genus Verticillium, representing 13 species of diverse econutritional groups (pathogens of insects, plants, mushrooms, nematodes and spiders, and saprobes), were examined by using sequences from the internal transcribed spacer (ITS) and small
A new fungal pathogen was isolated from rotten pomegranates collected from the orchards of different parts of Maharashtra. The pathogen was morphologically identified as Chaetomella raphigera followed by sequencing of ITS and D1/D2 hypervariable region of LSU (28S) of rRNA gene. The pathogen
Summary Erwinia carotovora ssp. carotovora (Ecc) possesses hrpN(Ecc)[hrp = gene for hypersensitive reaction (HR) and pathogenicity], the structural gene for Harpin(Ecc), the inducer of the HR-like response and genes for the type III secretion system. In Ecc, RsmA, an RNA-binding protein responsible
OBJECTIVE Isolation and characterization of a bacterial isolate (strain FP10) from banana rhizosphere with innate potential as fungal antagonist and microbial adjuvant in micropropagation of banana. RESULTS Bacterium FP10 was isolated from the banana rhizosphere and identified as Pseudomonas

Genomic, Network, and Phylogenetic Analysis of the Oomycete Effector Arsenal.

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The oomycetes are a class of microscopic, filamentous eukaryotes within the stramenopiles-alveolate-Rhizaria (SAR) supergroup and include ecologically significant animal and plant pathogens. Oomycetes secrete large arsenals of effector proteins that degrade host cell components, manipulate host

Structural biology of pectin degradation by Enterobacteriaceae.

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CONCLUSIONS Pectin is a structural polysaccharide that is integral for the stability of plant cell walls. During soft rot infection, secreted virulence factors from pectinolytic bacteria such as Erwinia spp. degrade pectin, resulting in characteristic plant cell necrosis and tissue maceration.

Polygalacturonase gene pgxB in Aspergillus niger is a virulence factor in apple fruit.

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Aspergillus niger, a saprophytic fungus, is widely distributed in soil, air and cereals, and can cause postharvest diseases in fruit. Polygalacturonase (PG) is one of the main enzymes in fungal pathogens to degrade plant cell wall. To evaluate whether the deletion of an exo-polygalacturonase gene
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