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Transcription of a sorghum phosphoenolpyruvate carboxylase gene in transgenic tobacco leaves: maturation of monocot PRE-mRNA by dicot cells.

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The expression of a Sorghum vulgäre gene encoding the phosphoenol-pyruvate carboxylase involved in C4 photosynthesis has been studied after introduction into tobacco. Northern blot analysis of poly(A) mRNA from green leaves demonstrated i) the efficiency of this monocot promoter, ii) the

Stomatal Behavior and Water Status of Maize, Sorghum, and Tobacco under Field Conditions: II. At Low Soil Water Potential.

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Diurnal changes in the vertical profiles of irradiance incident upon the adaxial leaf surface (I), leaf resistance (r(1)), leaf water potential (psi), osmotic potential (pi), and turgor potential (P) were followed concurrently in crops of maize (Zea mays L. cv. Pa602A), sorghum (Sorghum bicolor [L.]

Stomatal Behavior and Water Status of Maize, Sorghum, and Tobacco under Field Conditions: I. At High Soil Water Potential.

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Diurnal changes in the vertical profiles of irradiance incident upon the adaxial leaf surface (I), stomatal resistance (r(s)), leaf water potential (psi), osmotic potential (pi), and turgor potential (P) were followed concurrently in crops of maize (Zea mays L. var. Pa 602A), sorghum (Sorghum

Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in Dhurrin biosynthesis.

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Novel cyanogenic plants have been generated by the simultaneous expression of the two multifunctional sorghum (Sorghum bicolor [L.] Moench) cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco (Nicotiana tabacum cv Xanthi) and Arabidopsis under the regulation of the constitutive 35S promoter.

Transfer of the cytochrome P450-dependent dhurrin pathway from Sorghum bicolor into Nicotiana tabacum chloroplasts for light-driven synthesis.

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Plant chloroplasts are light-driven cell factories that have great potential to act as a chassis for metabolic engineering applications. Using plant chloroplasts, we demonstrate how photosynthetic reducing power can drive a metabolic pathway to synthesise a bio-active natural product. For this

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice.

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The type II CRISPR/Cas system from Streptococcus pyogenes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish and human cells. Here, we describe adaptations of these systems

Multivariate statistical models of metabolomic data reveals different metabolite distribution patterns in isonitrosoacetophenone-elicited Nicotiana tabacum and Sorghum bicolor cells.

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Isonitrosoacetophenone (INAP, 2-keto-2-phenyl-acetaldoxime) is a novel inducer of plant defense. Oxime functional groups are rare in natural products, but can serve as substrates depending on existing secondary pathways. Changes in the metabolomes of sorghum and tobacco cells treated with INAP were

Callus formation and differentiation in tissue cultures of normal and cytoplasmic male sterile sorghum, pepper, sunflower, and tobacco.

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YSK2 Type Dehydrin (SbDhn1) from Sorghum bicolor Showed Improved Protection under High Temperature and Osmotic Stress Condition.

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YSK2 type dehydrin from Sorghum bicolor (SbDhn1) showed a high level of transcript accumulation when subjected to high temperature and osmotic stress. The high transcript level occurring in such stress situation might lead to a protective effect; though the exact mechanism by which this is achieved

A chloroplast DNA deletion located in RNA polymerase gene rpoC2 in CMS lines of sorghum.

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Fertile lines of sorghum (Sorghum bicolor) were shown to differ from cytoplasmic male sterile (CMS) lines by the presence of a 3.8 kb HindIII chloroplast DNA fragment in the former and a smaller (3.7 kb) fragment in the latter. DNA/DNA hybridization studies showed that these two fragments are

Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions.

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Sorghum is one of the world's most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum's growing season and increases yield in temperate regions. However, sorghum's sensitivity to low soil temperatures adversely

Host Suitability of Grain Sorghum Cultivars to Meloidogyne spp.

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Grain sorghum cultivars (Funk G-499GBR, Funk G-611, Funk G-522A, Funk G-522DR, Coker 7723, Coker 7675, Coker 7623, Pioneer B815, Pioneer 8222, Pioneer 8272) were evaluated in the greenhouse for resistance to populations of Meloidogyne incognita race 3, M. arenaria race 2, and M. javanica from South

First Report of Pantoea agglomerans Causing Leaf Blight and Vascular Wilt in Maize and Sorghum in Mexico.

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Zea mays and Sorghum bicolor are important crops for animal and human nutrition worldwide. In the Central Highland Valley of Mexico, both crops are extremely important, and research is aimed toward increasing yield, disease resistance, and crop adaptation from 1,900- to 2,700-m elevation. In a

Map-based cloning and expression analysis of BMR-6 in sorghum.

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Brown midrib mutants in sorghum are associated with reduced lignin content and increased cell wall digestibility. In this study, we characterized a bmr-6 sorghum mutant, which shows reddish pigment in the midrib and stem after the fifth-leaf stage. Compared to wild type, Kalson lignin content of

An efficient and improved method for virus-induced gene silencing in sorghum.

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BACKGROUND Although the draft genome of sorghum is available, the understanding of gene function is limited due to the lack of extensive mutant resources. Virus-induced gene silencing (VIGS) is an alternative to mutant resources to study gene function. This study reports an improved and efficient

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