Antagonist-Elicited Cannabis Withdrawal

- Eligibility Crtieria for Canabis Users

INCLUSION CRITERIA:

1. 18 to 45 years of age;

2. Cannabis use of at least one year with a typical pattern of daily use for the three months prior to unit admission;

3. A urine sample positive for cannabinoids in the 30 days prior to study enrollment;

4. Blood pressure (BP) and heart rate (HR) at or below the following values while sitting after 5 minutes' rest: Systolic BP (SBP) 140 mm Hg, diastolic BP (DBP) 90 mm Hg,

heart rate (HR) 100 bpm;

5. 12-lead standard ECG and 3-minute rhythm strip without clinically relevant abnormalities and within the limits defined for the protocol in Appendix 2;

6. Peripheral veins suitable for repeated venipuncture and placement and maintenance of an IV catheter;

7. Ability to swallow capsules;

8. Ability to communicate well with the investigators and to comply with study requirements;

9. Women of childbearing potential must have a negative serum beta-human chorionic gonadotropin (beta-HCG) pregnancy test prior to study enrollment. All volunteers must use an intra-uterine device or hormonal contraception during the entire study period and for 1 month after. Women who are postmenopausal for more than 12 months or who were sterilized more than 3 months ago are not considered to have childbearing potential;

10. Estimated IQ greater than or equal to 85 determined by the Wechsler Abbreviated Scale of Intelligence

11. Right-handed (Experiment II only).

EXCLUSION CRITERIA:

1. History or presence of any clinically significant disease state, as detected by history, physical examination, and/or laboratory tests, that might put the subject at increased risk of adverse events or that might interfere with the absorption, distribution, metabolism, or excretion of study drugs;

2. Current physical dependence on any substance other than cannabis, nicotine, or caffeine;

3. Positive serological tests for syphilis or HIV infection;

4. Positive purified protein derivative (PPD) test in the absence of a negative chest Xray;

5. Consumption of an investigational drug within 90 days prior to study drug administration;

6. Consumption of any drug at a dose known to have a well-defined potential for toxicity to a major organ system within 3 months prior to study drug administration;

7. Symptoms of a clinically significant1 illness within two weeks prior to study drug administration;

8. History of a clinically significant1 adverse event associated with cannabis intoxication or withdrawal;

9. History of epileptic seizures or head trauma with loss of consciousness greater than three minutes;

10. History of psychosis or any current DSM-IV axis I disorder (other than cannabis, caffeine or nicotine dependence, or simple phobia);

11. Donation of more than 500 mL of blood within 30 days of study drug administration;

12. Regular use of alcohol (greater than or equal to 6 standard drinks per day) four or more times per week in the month prior to study entry;

13. If female, pregnant or nursing;

14. ADHD Screening Rating Scale score greater than or equal to 24 on either the A or B subscale;

15. Allergy to sesame seed oil (ingredient in dronabinol capsules);

16. Currently interested in or participating in drug abuse treatment, or participated in drug abuse treatment within 60 days preceding study enrollment.

17. Claustrophobia that precludes being able to tolerate an fMRI session (Experiment II only).

18. Magnetizable metal on or within the body that cannot be removed (Experiment II only).

Eligibility Criteria for Controls

The goal of the eligibility criteria for control subjects is to obtain a group approximately comparable to the cannabis users in terms of age, sex, ethnicity, and intelligence.

INCLUSION CRITERIA:

1. 18 to 45 years of age;

2. BP and HR at or below the following values while sitting after five minutes' rest: SBP 140 mm Hg, DBP 90 mm Hg, HR 100 bpm;

3. Ability to communicate well with the investigator and to comply with study requirements;

4. If female with reproductive potential, must be using a reliable method of birth control

5. Estimated IQ greater than or equal to 85 determined by the Wechsler Abbreviated Scale of Intelligence;

6. Right-handed

EXCLUSION CRITERIA:

1. History or presence of any clinically significant disease state, as detected by history, physical examination, and/or laboratory tests that might put the subject at increased risk of adverse events;

2. A positive urine test for any illicit drug at any time during screening or study participation;

3. Current physical dependence on any substance other than nicotine or caffeine;

4. Self-reported lifetime use of any illicit drug other than cannabis;

5. Self-reported lifetime use of cannabis greater than ten times or use within the last two years;

6. Positive serological tests for syphilis or HIV infection;

7. History of epileptic seizures or head trauma with loss of consciousness greater than three minutes;

8. History of psychosis or any current DSM-IV axis I disorder (other than caffeine or nicotine dependence, or simple phobia);

9. Regular use of alcohol (greater than or equal to 6 standard drinks per day) four or more times per week in the month prior to study entry;

10. If female, pregnant or nursing;

11. ADHD Screening Rating Scale score greater than or equal to 24 on either the A or B subscale;

12. Claustrophobia that precludes being able to tolerate an fMRI session;

13. Magnetizable metal on or within the body that cannot be removed.