Gingival Crevicular Fluid Levels Of Monocyte Chemoattractant Protein-1
Maneno muhimu
Kikemikali
Maelezo
Patients with aggressive periodontitis (AgP) are diagnosed as rapid and severe periodontal destruction commonly in younger systemically healthy individuals (Armitage, 1999). Although periopathogenic bacteria are essential for initiation, this alone does not be responsible for the manifestation and severity of the clinical status/phenotype (Offenbacher et al., 2008), suggesting an imbalance between host and bacterial load (Kinane et al., 2007). Response to bacterial biofilm is determined by the host immune system (Mahanonda and Pichyangkul, 2007, Amano, 2010) in which genetic and enviromental factors modify and may get individuals more susceptible or resistant to the periodontal diseases (Kulkarni and Kinane, 2014).
The role of whether an impaired or a hyperinflammatory host response is responsible for the progression of aggressive periodontitis has been a topic of discussion. Regarding to this topic Shaddox et al. (2016) studied the whole blood specimens collected from LAgP subjects and stimulated with plaque sample generated from healthy or diseased sites. And as a result, LAgP subjects displayed hyperinflammatory response regardless of contents of bacterial stimulus. In an another research hyperinflammatory responders LAgP subjects presented the lowest reductions in clinical parameters, on the contrary low responders showed the highest reductions after treatment (Allin et al., 2016). Similarly Garrison and Nichols (1989) reported that hyper-inflammatory monocyte phenotype can be deterministic in periodontal destruction. In the view of the aforementioned findings, the aim of this study is to estimate GCF MCP-1 levels of healthy and AgP subjects and to compare MCP-1 levels between LAgP and GAgP to establish its predictive value/role for distinguishing LAgP and GAgP development.
A total of 160 subjects including, 80 AgP and 80 age and gender matched periodontally healthy (H) controls were recruited in this cross-sectional study. Clinical periodontal measurements including plaque index (PI), gingival index (GI), proping depth (PD) and clinical attachment loss (CAL) were performed. GCF samples were collected from 160 patients including 50 LAgP, 30 GAgP and 80 H. Volume of GCF samples were measured by an electronic device (Periotron 8000, OroFlow Inc.) and GCF MCP-1 was measured by an enzyme-linked immunosorbent assay "ELISA".
Tarehe
| Imethibitishwa Mwisho: | 09/30/2016 |
| Iliyowasilishwa Kwanza: | 10/05/2016 |
| Uandikishaji uliokadiriwa Uliwasilishwa: | 10/05/2016 |
| Iliyotumwa Kwanza: | 10/06/2016 |
| Sasisho la Mwisho Liliwasilishwa: | 10/05/2016 |
| Sasisho la Mwisho Lilichapishwa: | 10/06/2016 |
| Tarehe halisi ya kuanza kwa masomo: | 02/28/2011 |
| Tarehe ya Kukamilisha Msingi iliyokadiriwa: | 07/31/2012 |
| Tarehe ya Kukamilisha Utafiti: | 07/31/2012 |
Hali au ugonjwa
Uingiliaji / matibabu
Other: Gingival crevicular fluid (GCF) sample
Awamu
Vikundi vya Arm
| Mkono | Uingiliaji / matibabu |
|---|---|
| case (Aggressive periodontitis) Single intervention has been performed for each subject. Gingival crevicular fluid sample was obtained in conjunction with clinical measurements in this intervention. | |
| control (Periodontally healthy subjects) Single intervention has been performed for each subject. Gingival crevicular fluid sample was obtained in conjunction with clinical measurements in this intervention. |
Vigezo vya Kustahiki
| Zama zinazostahiki Kujifunza | 16 Years Kwa 16 Years |
| Jinsia Inastahiki Kujifunza | All |
| Njia ya sampuli | Probability Sample |
| Hupokea Wajitolea wa Afya | Ndio |
| Vigezo | Inclusion Criteria: - Periodontally Healthy control group (n: 80) had < 3mm PD, < 2 GI and no signs of interproximal attachment loss and a history of periodontal disease. - The AgP group included individuals diagnosed with localized AgP (LAgP) or generalized AgP (GAgP) who were otherwise healthy. Periodontal attachment loss ≥4 mm accompanied by interproximal bone loss without gingival recession not involving more than two permanent teeth, other than the first molars and incisors were diagnosed with LAgP (n: 50); patients with involvement of at least three teeth, other than the first molars and incisors with an attachment loss ≥4 mm accompanied by interproximal bone loss without gingival recession were diagnosed with GAgP (n: 30). Exclusion Criteria: - Patients who have any systemic diseases or conditions that could effect the periodontium - hepatitis and/or immunodeficiency virus infection - a history of periodontal treatment and antibiotic therapy within the 6 months - < 16 teeth in their mouth - Subjects who had smoked and were ongoing orthodontic treatment were excluded. |
Matokeo
Hatua za Matokeo ya Msingi
1. Determination of GCF MCP-1 levels in patients with AgP [1 year]
Hatua za Matokeo ya Sekondari
1. Measurement of clinical periodontal parameters in patients with AgP and compare between LAgP and GAgP [1 year]
