Antiinflammatory Effect of Dietary Protein Intake in Elderly People
Maneno muhimu
Kikemikali
Maelezo
Participants to be recruited, are aged 50 to 70y from the Healthy Aging Study (EHSREC10_45) who will attend on 4 test days (occasions)
Study Design: Single blind, randomised, repeated measures design with 2 independent factor (time x treatment)
Procedures:
Occasion 1 Subjects will undertake a preliminary assessment as per EHSREC 10_45 as follows; i. medical history and examination by a qualified medical doctor (~45 min); ii. provide a blood and urine sample to be evaluated (following analysis) by a qualified medical doctor;
Occasion 2 Subjects will then undertake, as per EHSREC 2013 01_12, an assessment of body composition, dietary intake and habitual physical activity as follows; iii. habitual food intake evaluated by a qualified dietitian (~45 min); iv. body composition (~45 min) v. habitual physical activity level (PAL) quantified using the European Physical Activity Questionnaire (EPAQ-2; ~ 15 min).
Occasion 3/4
Subjects will then undertake two controlled feeding trials, each trial separated by approximately 7 days as follows:
Subjects will report to the Metabolic Laboratory (PG-052b), Physical Education and Sports Sciences (PESS) Building following an overnight fast.
(i) A cannula will be inserted into a peripheral arm vein by a qualified clinician or phlebotomist;
(ii) a 10ml blood sample will be withdrawn and aliquots generated for subsequent analysis; (iii) Following a 15 min rest period subjects will be given in randomised order either control (CONTROL) - a placebo nutrient drink (per kg of body mass: 0.33g protein with no anti-inflammatory bioactivity; energy ~ 160 kcal in 500ml water) or nutrient (NUTRIENT) - a nutrient drink (per kg of body mass: 0.33g protein with confirmed anti-inflammatory bioactivity; energy ~ 160 kcal in 500ml water) to be consumed within a 10 minute period.
CONTROL and NUTRIENT will be reversed for the 2nd trial in randomised order.
(iv) Whilst remaining sitting at rest (reading, undertaking computer work etc.) further blood samples will be withdrawn at 0, 15, 30, 45, 60 75, 90, 120 and 180 minutes post feeding.
Total blood draw = 100ml per trial.
Data Analysis: (i) Shapiro-Wilk test of normality, then (ii) repeated measures analysis either by ANOVA(R) if normal or Friedman Test if non-normally distributed.
Tarehe
| Imethibitishwa Mwisho: | 06/30/2017 |
| Iliyowasilishwa Kwanza: | 02/11/2016 |
| Uandikishaji uliokadiriwa Uliwasilishwa: | 04/26/2016 |
| Iliyotumwa Kwanza: | 05/02/2016 |
| Sasisho la Mwisho Liliwasilishwa: | 07/30/2017 |
| Sasisho la Mwisho Lilichapishwa: | 07/31/2017 |
| Tarehe halisi ya kuanza kwa masomo: | 01/31/2016 |
| Tarehe ya Kukamilisha Msingi iliyokadiriwa: | 04/07/2017 |
| Tarehe ya Kukamilisha Utafiti: | 04/07/2017 |
Hali au ugonjwa
Uingiliaji / matibabu
Dietary Supplement: CONTROL
Dietary Supplement: NUTRIENT
Awamu
Vikundi vya Arm
| Mkono | Uingiliaji / matibabu |
|---|---|
| Placebo Comparator: CONTROL A placebo nutrient drink with no anti-inflammatory bioactivity (measure by cell bioassay in vitro) Dose provided per kg of body mass: 0.33g protein mixed in 500ml of water as a protein 'shake' Energy ~ 160 kcal | Dietary Supplement: CONTROL Following an overnight fast, participants submit to cannulation of a superficial arm vein from which are drawn serial samples of blood prior to and for 3 hours following ingestion of a prescribed protein 'shake' |
| Active Comparator: NUTRIENT A nutrient drink with confirmed anti-inflammatory bioactivity (measured by cell bioassay in vitro) Dose provided per kg of body mass: 0.33g protein mixed in 500ml of water as a protein 'shake' Energy ~ 160 kcal | Dietary Supplement: NUTRIENT Following an overnight fast, participants submit to cannulation of a superficial arm vein from which are drawn serial samples of blood prior to and for 3 hours following ingestion of a prescribed protein 'shake' |
Vigezo vya Kustahiki
| Zama zinazostahiki Kujifunza | 50 Years Kwa 50 Years |
| Jinsia Inastahiki Kujifunza | All |
| Hupokea Wajitolea wa Afya | Ndio |
| Vigezo | Inclusion Criteria: - Men and Women - Aged 50 to 70y - Conducted by a medical doctor and based on Grieg et al. 1994 to be 'healthy' (i.e. 'disease-free') free-living and fully mobile Exclusion Criteria: - Age <50 or > 70y. - Contraindication identified by medical doctor based on Grieg et al. 1994 definition of 'healthy' (i.e. 'disease-free') free-living and fully mobile older subjects - Lactose intolerant or intolerance to dairy-based foods. |
Matokeo
Hatua za Matokeo ya Msingi
1. Interleukin 6 (IL-6) response of macrophage cells to stimulation with bacterial lipopolysaccharide (LPS) [All timepoints to 180 min following ingestion of NUTRIENT and CONTROL drinks]
2. Tumour necrosis factor alpha (TNF-alpha) response of macrophage cells to stimulation with bacterial lipopolysaccharide (LPS) [All timepoints to 180 min following ingestion of NUTRIENT and CONTROL drinks]
3. Interleukin-1 beta (IL1_beta) response of macrophage cells to stimulation with bacterial lipopolysaccharide (LPS) [All timepoints to 180 min following ingestion of NUTRIENT and CONTROL drinks]
