In Vivo Inhibition Profile of CYP2C9 by Pineapple Juice
Maneno muhimu
Kikemikali
Maelezo
For ovarian cancer, colorectal and gastric cancers presenting with peritoneal metastases, complete tumor removal at surgery is the most important independent prognostic factor. Consequently, accurate detection of tumors often compromising resectability, like extra-abdominal metastases, liver metastases, portal and superior mesenteric artery deposits and extensive intestinal serosal invasion is pivotal prior to treatment selection. Computed tomography (CT) has variable accuracy for staging, due to the difficult detection of low-contrast or small-sized peritoneal or nodal metastases. Fluorodeoxyglucose positron emission tomography/CT (FDG-PET/CT) improves detection of thoraco-abdominal lymphadenopathy and liver metastases, but inconsistently detects small (<5mm) peritoneal metastases. Therefore a diagnostic staging laparoscopy under general anesthesia is currently the necessary standard of clinical practice in addition to imaging for assessment of operability.
Whole body diffusion-weighted magnetic resonance imaging is researched at the department of radiology, University Hospitals Leuven in collaboration with the departments of abdominal surgery, oncologic surgery, oncologic gynaecology and digestive oncology. The technique holds high promise to combine a high accuracy in systemic thoraco-abdominal staging and peritoneal assessment of operability. Technological progress has enabled time-efficient WB-DWI with thin-slice-acquisition and multiplanar image reformatting. DWI depicts lesions by measuring water diffusion differences, correlating with cellular density. Tumors are depicted with high signal compared to background by combining a short-T1-inversion-time inversion recovery (STIR) prepulse - suppressing ascites, blood vessels, fat, bowel and visceral organs - and heavy diffusion weighting. However, due to contraction and mucosal cellularity, the bowel wall can show increased signal-intensity (SI), hampering the detection of serosal deposits. This is overcome by suppressing contractions by intravenous antispasmodic and by distending the bowel wall and suppressing the signal of bowel content by peroral pineapple juice which shows negative contrast properties due to the manganese-content. In a first pilot study in ovarian cancer at this center in 32 patients, an accuracy for detection of intestinal serosal metastases of 90% was reached by WB-DWI combined with peroral pineapple juice. As such, the pineapple juice plays a pivotal role as a peroral contrast in addition to WB-DWI for accurate peritoneal staging.
To date, the inhibitory potential of pineapple juice on cytochrome P450 2C9 activity has only been described in vitro in human microsomes. In this model, in which diclofenac and its metabolite 4-OH-diclofenac have been used as probes for CYP2C9 activity, it has been shown that pineapple juice is capable to inhibit CYP2C9 very potently (IC50 0.08%) in an irreversible manner. It has been suggested that the main effect is caused by bromelain, a 24-26 kDa cysteine protease enzyme present in pineapple juice. The intestinal absorption of intact bromelain after oral intake has been described in 19 healthy men, which is surprising as the adult intestinal epithelium has traditionally been described as non-permeable to proteins. The (limited) absorption is thought to occur via the paracellular route, which could explain that the catalytic activity bromelain is preserved following absorption into the blood circulation. Although no effects of bromelain on CYP2C9 activity are expected in vivo (due to low oral bioavailability), no in vivo trials have been undertaken to elucidate if pineapple juice, and more specifically bromelain, is capable of inhibiting intestinal and, more importantly, hepatic CYP2C9 in a clinically relevant manner.
The in vivo inhibitory profile of CYP2C9 by pineapple juice will be evaluated in this study in 10 healthy volunteers, by examining the impact on the area-under-the-curves (AUCs) of diclofenac and its metabolite 4-OH diclofenac.
Tarehe
Imethibitishwa Mwisho: | 06/30/2012 |
Iliyowasilishwa Kwanza: | 07/19/2012 |
Uandikishaji uliokadiriwa Uliwasilishwa: | 07/19/2012 |
Iliyotumwa Kwanza: | 07/24/2012 |
Sasisho la Mwisho Liliwasilishwa: | 07/19/2012 |
Sasisho la Mwisho Lilichapishwa: | 07/24/2012 |
Tarehe halisi ya kuanza kwa masomo: | 08/31/2012 |
Tarehe ya Kukamilisha Msingi iliyokadiriwa: | 09/30/2012 |
Tarehe ya Kukamilisha Utafiti: | 09/30/2012 |
Hali au ugonjwa
Uingiliaji / matibabu
Dietary Supplement: diclofenac with pineapple juice
Awamu
Vikundi vya Arm
Mkono | Uingiliaji / matibabu |
---|---|
No Intervention: diclofenac without pineapple juice single dose of diclofenac 25 mg without pre-exposure to pineapple juice | |
Active Comparator: diclofenac with pineapple juice single dose of diclofenac 25 mg with pre-exposure to pineapple juice | Dietary Supplement: diclofenac with pineapple juice |
Vigezo vya Kustahiki
Zama zinazostahiki Kujifunza | 18 Years Kwa 18 Years |
Jinsia Inastahiki Kujifunza | All |
Hupokea Wajitolea wa Afya | Ndio |
Vigezo | Inclusion Criteria: - adult healthy volunteers Exclusion Criteria: - younger than 18 yrs - older than 60 yrs - pregnant or lactating women - medical history of gastric or duodenal ulcers, gastro-oesofageal reflux disease, dyspepsia, asthma, any allergy to NSAIDS - patients taking co-medication |
Matokeo
Hatua za Matokeo ya Msingi
1. a) AUC 4-OH-diclofenac / AUC diclofenac quantified in plasma, on days 1 (without pineapple juice) and 11 (after pretreatment with pineapple juice) [day 1 and day 11]
Hatua za Matokeo ya Sekondari
1. (b) AUC 4-OH-diclofenac/ AUC diclofenac quantified in urine, on days 1 (without pineapple juice) and 11 (after pretreatment with pineapple juice) [day 1 and day 11]
Hatua Nyingine za Matokeo
1. c) Bromelain activity quantified in plasma, measured on days 1 and 11 [day 1 and day 11]