Polyphenols and Overfeeding

Watumiaji waliosajiliwa tu ndio wanaweza kutafsiri nakala

Ingia / Ingia

Kiungo kimehifadhiwa kwenye clipboard

HaliImekamilika

Wadhamini

Hospices Civils de Lyon

JARIBIO LA KLINIKI: NCT02145780

BioSeek: nct02145780

Maneno muhimu

Kikemikali

Obesity is a complex and multifactorial disease representing a major public health challenge. Indeed, overweight and obese people carry a major risk of developing insulin resistance and type 2 diabetes mellitus. It has been demonstrated that polyphenols could be used to counteract some of the mechanisms involved in the generation of insulin resistance. The model of overfeeding represents a very good study model of the metabolic complications of obesity, since it has been shown to induce a transient state of insulin resistance in the human.

The purpose of this research is to study the effects of a overfeeding (+50% of daily caloric needs over 31 days) with or without polyphenols supplementation (2g/days over 31 days) on insulin sensitivity measured in the setting of a hyperinsulinemic euglycemic clamp and on post prandial partitioning of exogenous lipids after a test meal.

During 31 days, the volunteers will consume chocolate bars, chips, chocolate breads and cola in addition to their usual diet and will be supplemented with 2g of grape polyphenols extract or with placebo.

Tarehe

Imethibitishwa Mwisho:	07/31/2017
Iliyowasilishwa Kwanza:	05/12/2014
Uandikishaji uliokadiriwa Uliwasilishwa:	05/19/2014
Iliyotumwa Kwanza:	05/22/2014
Sasisho la Mwisho Liliwasilishwa:	08/21/2017
Sasisho la Mwisho Lilichapishwa:	08/22/2017
Tarehe halisi ya kuanza kwa masomo:	01/31/2014
Tarehe ya Kukamilisha Msingi iliyokadiriwa:	04/30/2017
Tarehe ya Kukamilisha Utafiti:	04/30/2017

Hali au ugonjwa

Overfeeding

Uingiliaji / matibabu

Dietary Supplement: 2g of grape polyphenol extract supplement

Dietary Supplement: 2g of placebo (lactose)

Awamu

Vikundi vya Arm

Mkono	Uingiliaji / matibabu
Experimental: 2g of grape polyphenol extract supplement Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.	Dietary Supplement: 2g of grape polyphenol extract supplement Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.
Placebo Comparator: 2g of placebo (lactose) Men will have to consume daily 2g of placebo during the 31 days of overfeeding.	Dietary Supplement: 2g of placebo (lactose) Men will have to consume daily 2g of placebo during the 31 days of overfeeding.

Vigezo vya Kustahiki

Zama zinazostahiki Kujifunza	18 Years Kwa 18 Years
Jinsia Inastahiki Kujifunza	Male
Hupokea Wajitolea wa Afya	Ndio
Vigezo	Inclusion Criteria: - Male - Aged from 18 to 55 years - Body Mass Index of 23 to 27 kg/m² - Normal blood pressure - Fasting glycemia < 7 mmol/L - CRPus < 10mg/L - Triglycerides ≤ 3mmol/L Exclusion Criteria: - Medical or surgical history which may affect the results (renal -cardiovascular - hepatic- endocrine-inflammatory diseases) - Subjects under treatment which may interfere with the measured parameters - Eating disorder - Intensive sportive activity - Dairy products allergy or intolerance

Matokeo

Hatua za Matokeo ya Msingi

1. Peripheral insulin sensitivity [During 31 days]

Volunteers will have peripheral and hepatic insulin sensitivity determined by using a two-stage hyperinsulinemic euglycemic clamp with stable isotopically labeled tracer infusion.

Hatua za Matokeo ya Sekondari

1. Hepatic insulin sensitivity [before and after the 31 days of overfeeding]

Endogenous glucose production (EGP) will be determined during the last 30 minutes of the low-dose insulin infusion.

2. Change in genes expression in adipose and muscle tissues [Before and after the 31 days of overfeeding]

RT-PCR

3. Anthropometry, Fat quantification and Abdominal fat distribution [During 31 days]

Weight, height, waist and hip circumferences, fat mass using dual x-ray absorptiometry (DEXA) and abdominal fat distribution using resonance magnetic imaging (RMI).

4. Fasting and Postprandial partitioning of exogenous lipid [Fasting and during all the postprandial period (0-300 minutes)]

13C enrichment will be measured in blood samples and breath test during a test meal performed before and after the 31 days of overfeeding.

5. Modification in intestinal microbiota [Before and after the 31 days of overfeeding]

Feces analyses

6. Energy expenditure and substrate oxidation with indirect calorimetry [Fasting and during all the postprandial period (0-300 minutes)]

Energy metabolism will be measured before and after the 31 days of overfeeding.

Polyphenols and Overfeeding

Maneno muhimu

polyphenol

kisukari aina ya 2

kisukari

insulin resistance

kunona