The Effect of Intravenous Nutrition in Patients Undergoing Abdominal Surgery
Maneno muhimu
Kikemikali
Maelezo
1. Goals The overall goal of perioperative nutrition support is to abolish protein wasting and to promote anabolic processes by directing amino acids into protein synthesis rather than oxidation. Protein repletion and enhancement of anabolism appear to be particularly important in cancer patients who enter major abdominal surgery in a catabolic state.
2. Objectives The objectives of this research program are
- to examine whether the anabolic effects of hypocaloric nutrition depend on the degree of catabolism before the operation and thus identify patients who benefit the most from perioperative nutrition support (study I)
- to investigate whether excluding glucose from hypocaloric nutrition, i.e. infusing an isonitrogenous amount of amino acids without glucose avoids hyperglycemia and, thus, accentuates the patient's anabolic response to feeding (study II).
In order to confirm the validity of our assumptions we will perform two consecutive studies in two distinct patient populations. For the assessment of the patients' catabolic state and obtaining insight into the biochemical mechanisms, whereby the effects of nutrition are mediated, stable isotope tracer kinetics will be applied. Using primed continuous infusions of L-[1-13C]leucine and [6,6-2H2]glucose we will quantitate the whole body dynamics of protein and glucose metabolism, i.e. protein breakdown, amino acid oxidation, protein synthesis, glucose production and glucose uptake before and after surgery. A positive protein balance (difference between protein synthesis and protein breakdown) will be used as an indicator of anabolism. Anabolic processes at the organ level (liver, muscle), i.e. fractional synthesis rates of the acute phase proteins albumin and fibrinogen and muscle protein synthesis will be determined using L-[2H5]phenylalanine infusions. Skeletal muscle protein catabolism will be characterized by measuring the mRNA expression of ubiquitin and two of its key ligases in muscle (MAFbx/atrogen-1 and MuRF-1).
Tarehe
| Imethibitishwa Mwisho: | 07/31/2011 |
| Iliyowasilishwa Kwanza: | 08/08/2011 |
| Uandikishaji uliokadiriwa Uliwasilishwa: | 08/09/2011 |
| Iliyotumwa Kwanza: | 08/10/2011 |
| Sasisho la Mwisho Liliwasilishwa: | 08/09/2011 |
| Sasisho la Mwisho Lilichapishwa: | 08/10/2011 |
| Tarehe halisi ya kuanza kwa masomo: | 10/31/2009 |
| Tarehe ya Kukamilisha Msingi iliyokadiriwa: | 05/31/2011 |
| Tarehe ya Kukamilisha Utafiti: | 06/30/2011 |
Hali au ugonjwa
Uingiliaji / matibabu
Other: Glucose and amino acids
Other: Amino acids only
Awamu
Vikundi vya Arm
| Mkono | Uingiliaji / matibabu |
|---|---|
| Experimental: Glucose and amino acids Perioperative nutrition with glucose and amino acids | Other: Glucose and amino acids Glucose and amino acids intravenously starting 20 hours before the operation until the second postoperative day. Glucose provides 50% and amino acids 20% of each patient's measured resting energy expenditure. |
| Active Comparator: Amino acids only Perioperative nutrition with amino acids only | Other: Amino acids only Amino acids intravenously starting 20 hours before the operation until the second postoperative day. Amino acids providing 20% of each patient's measured resting energy expenditure. |
Vigezo vya Kustahiki
| Zama zinazostahiki Kujifunza | 18 Years Kwa 18 Years |
| Jinsia Inastahiki Kujifunza | All |
| Hupokea Wajitolea wa Afya | Ndio |
| Vigezo | Inclusion Criteria: - American Society of Anesthesiologists <3 - colorectal surgery for non-metastatic colorectal carcinoma including right and left hemicolectomy, transverse, subtotal and total colectomy, sigmoid resection - ability to give informed consent Exclusion Criteria: - signs of severe malnutrition or obesity: body mass index <18 or >25 >10% involuntary body weight loss over the preceding 6 months serum albumin <21 g/L - significant cardiorespiratory, hepatic, renal and neurological disease - ingestion of drugs known to affect protein, glucose and lipid metabolism (for example steroids) - musculoskeletal or neuromuscular disease - severe anemia (hemoglobin <10 g/dL) - pregnancy - history of severe sciatica or back surgery or other conditions which - contraindicate the use of epidural catheters |
Matokeo
Hatua za Matokeo ya Msingi
1. protein balance [two days after surgery]
Hatua za Matokeo ya Sekondari
1. albumin synthesis [two days after surgery]
2. fibrinogen synthesis [two days after surgery]
3. total plasma protein synthesis [two days after surgery]
4. mRNA expression of ubiquitin [two days after surgery]
