[Determination of vetatramine in Veratrum nigrum by HPLC-ELSD].
Maneno muhimu
Kikemikali
OBJECTIVE
To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.
METHODS
The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).
RESULTS
The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.
CONCLUSIONS
The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.
