Micropropagation of Cordyline terminalis.
Maneno muhimu
Kikemikali
This protocol describes an efficient and rapid method for large-scale multiplication of Cordyline terminalis in a cost-effective manner. Actively growing shoot tips were selected as explants. Murashige and Skoog (MS) basal medium was supplemented with different plant growth regulators at various developmental stages of C. terminalis. The highest percentage of regeneration (95 ± 2.8) and average number of shoot buds (60.2 ± 4.4) per explant were obtained in medium containing 80 mg /L adenine sulfate (AdSO(4)), 2 mg/L 6-benzyladenine (BA), and 0.1 mg/L indole-3-acetic acid (IAA). Thousands of micropropagated plants were produced within 4-5 months using this protocol.