3 matokeo
A protease has been purified from the sarcocarp of Benincasa hispida (Thunb.) Cogn. var. Ryukyu by two steps of chromatography. Its M(r) was estimated by SDS-PAGE to be about 67,000. The enzyme was strongly inhibited by diisopropyl fluorophosphate, but not by EDTA and cysteine protease inhibitors.
A pathogenesis-related (PR) protein was purified from the seeds of Benincasa hispida, which is a medicinal plant and a member of the Cucurbitaceae family. Purification was achieved by using a procedure consisting of an acid treatment step followed by two chromatography steps. The protein is a basic
Two squash family protease inhibitors were obtained from wax gourd (Benicasa hispida [Thunb] Cogn.). Even though they were distinctly separated by reversed-phase chromatography, the amino acid sequences of two inhibitors were identical. Both inhibitors were converted into each other, perhaps due to