A group of genes including GhCIR1 from cotton (Gossypium hirsutum), and AtCIR1 and AtCIR2 from Arabidopsis thaliana promote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.

Ji; Lianghui

Cai; Lin

Chua; Nam-Hai

Temasek Life Sciences Laboratory

Plant homeodomain protein-encoding genes and their uses

It is to provide a novel molybdenum ion transporter MoTR1 gene responsible for molybdenum transport for the first time in plants, enabling to promote effectively molybdenum absorption from the environment or molybdenum transport in vivo. By QTL analysis of Arabidopsis thaliana accessions Col-0 and Ler, it was found that QTL which dominates Mo concentration in leaves was present on the chromosome No: 2. In the present invention, the region in which the causal gene is present was limited to 172 kb, by a genetic analysis. In the region, the gene At2g25680 having a domain common to sulfate ion transporter, while its function is not revealed was present. Thus, knockout strains of 2 separate lines in which a foreign gene fragment (T-DNA) was introduced at At2g25680 were obtained, Mo concentration in leaves was measured, and MoTR1 gene was identified.

common/arabidopsis thaliana

Plant homeodomain protein-encoding genes and their uses

Molybdenum transporter gene and use thereof

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