Immature oocytes are culture in the new capacitation-IVM system.

Immature oocytes are cultured in the standard IVM system.

Infertility, Female

Immature oocytes were plated into a 4-well dish (Nunc, Denmark) at 10 COCs/well using CAPA medium (Medicult IVM medium; Origio, Denmark supplemented with 1 mIU/mL rFSH, 5 ng/mL insulin, 10 nM estradiol, 10 mg/mL human serum albumin [SAGE, Denmark] and 25 nM CNP under oil for 24 h at 37°C, 6% carbon dioxide in air). After 24 hours, COCs were washed and transferred into IVM medium (Origio, Denmark) containing 5 ng/mL insulin, 10 nM estradiol, 100 ng/mL human recombinant amphiregulin and 100 mIU/mL rFSH, and incubated under oil for 30 h at 37°C, 6% carbon dioxide in air.

CAPA-IVM

Immature oocytes are plated into a 4-well dish at 10 COCs/well using IVM medium supplemented with 75 mIU/mL recombinant FSH (Merck, Switzerland), 100 mIU/mL hCG (MSD, USA), 0.01 mg/mL growth hormone (Merck, Switzerland) and 10 mg/mL human serum albumin (SAGE, Denmark). COCs were incubated for 30 hours at 37°C, 6% carbon dioxide in air.

Standard-IVM

My Duc Hospital

CAPA-IVM Versus STANDARD IVM

Research Study to Evaluate Capacitation Culture (CAPA) Followed by in Vitro Maturation (IVM) of Human Oocytes: A Randomized Pilot Study

Live birth is defined as the birth of at least one newborn after 24 weeks' gestation that exhibits any sign of life (twin will be a single count). For the timing of this occur, ongoing pregnancy will be used, i.e. ongoing pregnancy at 12 weeks will be used in calculations, conditional on the fact that this ongoing pregnancy results in live birth.

Pregnancy with detectable heart rate at 12 weeks' gestation

Pregnancy with detectable gestational sac at 7 weeks' gestation

The number of oocyte identified after oocyte retrieval

Rate of mature oocytes on culture oocytes

Number of fertilized oocyte after sperm insemination

Mỹ Đức Hospital

Randomized Clinical Trial
Patients are randomized to either CAPA-IVM or STANDARD-IVM using block randomization by an independent study coordinator using a computer-generated random list (block size 4) on the second day of their periods.

Bariatric Surgery Candidate

Pregnancy

UZ Leuven

bAriatric sUrgery Registration in wOmen of Reproductive Age

Bariatric Surgery Registration in Women of Reproductive Age

Weight at birth minus weight at start of pregnancy

Preterm (<37w of gestation) vs a term (37-42w of gestation) delivery

bowel obstruction, strangulation, internal herniation

pregnancy-induced hypertension, (pre-)eclampsia

Weight of newborn baby (<2.5kg small-for-gestational age; >4kg macrosomia)

quality of life assessed by the Moorehead-Ardelt Quality of Life Questinnaire II

Breast milk fat content assessed by the Human Milk Analyzer

Pattern and duration of menstrual cycle and contraceptive use assessed by a self-developed questionnaire

Level of physical activity (household, work, free time, sports) assessed by the Kaiser Physical Activity Survey

Dietary intake assessed by a semi-structured 3 day estimated dietary record method (3 day food diary)

sleeping habits (week and week-end) assessed by a self-developed questionnaire

Assessed by a structured questionnaire by Guelinckx et al. (2011): initiation and duration of breastfeeding, exclusively breastfeeing, artificial feeding

State and Trait anxiety assessed by the State-Trait Anxiety Inventory

depressive feelings assessed by the Edinburgh Postnatal Depression Scale;

drinking habits assessed by the Alcocol Abuse Disorder Identification Test (AUDIT-c)

Smoking habits (amount of sigarets, changes during pregnancy) assessed by a self-developed questionnaire

Sexual activity is assessed by a self-developed questionnaire

red blood cells, hematocrit, hemoglobin, MCV, MCH, MCHC assessed by venipuncture

Vitamin A serum level assessed by venipuncture

Vitamin 25-OH-vitamin D serum level assessed by venipuncture

Vitamin K1 serum level assessed by venipuncture

Vitamin B12 serum level assessed by venipuncture

Folate (serum and red blood cells) assessed by venipuncture

prothrombin time, activated partial tromboplastin time and coagulation factors assessed by venipuncture

Fasten glucose level assessed by venipuncture

Zinc serum level assessed by venipuncture

Iron, ferritin, transferrin (saturation) levels assessed by venipuncture

Breast milk fat protein assessed by the Human Milk Analyzer

Breast milk carbohydrate content assessed by the Human Milk Analyzer

Breast milk energy content assessed by the Human Milk Analyzer

vaginal, cesarean section, instrumental delivery

Apgar score (appearance, pulse, grimase response, activity, respiration) assessed by the medical staff

Weight measured by the medical staff to 1) calculate the body mass index and 2) to report weight loss (after surgery) or weight gain (during pregnancy)

Maternal age (birth date, age) assessed by online questionnaire

Belgium versus other assessed by online questionnaire

Family status (living together with or without children, living seperate, living with parents) assessed by online questionnaire

Educational level (until 18 yr, +18yr high school, +18yr university or higher) assessed by online questionnaire

Profession (working full/half time, studying, unemployed) assessed by online questionnaire

Marietal status (married, living together, in a relation, divorced, widow) assessed by online questionnaire

oligomenorrhea/albumin

CAPA-IVM Versus STANDARD IVM

bAriatric sUrgery Registration in wOmen of Reproductive Age

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