pinus/nikotini

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Ukurasa 1 kutoka 57 matokeo

Thymidylate synthase activity from chlamydomonas cells and cultured tissues of Nicotiana, pinus, and daucus.

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Ingia / Ingia

Prior preparation of N(5),N(10)-methylenetetrahydrofolate from dl-tetrahydrofolate and [(14)C]formaldehyde resulted in an improved assay for thymidylate synthase. Although preparations from tobacco seedlings and cotton root tips (0.25 centimeter) were inconsistent with respect to enzyme activity,

Characterization of Three L-Asparaginases from Maritime Pine (Pinus pinaster Ait.).

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Ingia / Ingia

Asparaginases (ASPG, EC 3.5.1.1) catalyze the hydrolysis of the amide group of L-asparagine producing L-aspartate and ammonium. Three ASPG, PpASPG1, PpASPG2, and PpASPG3, have been identified in the transcriptome of maritime pine (Pinus pinaster Ait.) that were transiently expressed in Nicotiana

Characterization of two laccases of loblolly pine (Pinus taeda) expressed in tobacco BY-2 cells.

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Ingia / Ingia

We previously showed that eight laccase genes (Lac 1-Lac 8) are preferentially expressed in differentiating xylem and are associated with lignification in loblolly pine (Pinus taeda) [Sato et al. (2001) J Plant Res 114:147-155]. In this study we generated transgenic tobacco suspension cell cultures

Abscisic acid in needles of Pinus cembra in relation to ozone exposure.

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Ingia / Ingia

Ozone pollution was analyzed in arolla pine (Pinus cembra L.) forests growing over two mountain ranges located in southern France by using specific ozone-sensitive tobacco plants as bio-indicators and a physico-chemical analyzer. Concentrations of abscisic acid (ABA) were determined in needles of

Exploring lignification in conifers by silencing hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase in Pinus radiata.

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Ingia / Ingia

The enzyme hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase (HCT) is involved in the production of methoxylated monolignols that are precursors to guaiacyl and syringyl lignin in angiosperm species. We identified and cloned a putative HCT gene from Pinus radiata, a coniferous gymnosperm

Seasonal variation of western white pine (Pinus monticola D. Don) foliage proteins.

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Ingia / Ingia

Recently, a western white pine protein, Pin m III, was shown to be associated with overwintering and frost hardiness of western white pine foliage. To examine whether Pin m III is directly involved in frost hardiness by functioning as an antifreeze protein, work is underway to clone the gene

Chloroplast-encoded chlB gene from Pinus thunbergii promotes root and early chlorophyll pigment development in Nicotiana tabaccum.

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Ingia / Ingia

Chlorophyll biosynthesis is catalyzed by two multi subunit enzymes; a light-dependent and a light-independent protochlorophyllide oxidoreductase. The light-independent enzyme consists of three subunits (ChlL, ChlN and ChlB) in photosynthetic bacteria and plastids in which the chlB gene encodes the

Duplication of the psbA gene in the chloroplast genome of two Pinus species.

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Ingia / Ingia

The psbA gene, encoding the D1 protein of photosystem II, was found to be duplicated in the chloroplast genome of two pine species, Pinus contorta and P. banksiana. Analysis of cloned overlapping restriction fragments of P. contorta chloroplast DNA showed that the two psbA genes have the same

Involvement of VNS NAC-domain transcription factors in tracheid formation in Pinus taeda.

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Ingia / Ingia

Vascular plants have two types of water-conducting cells, xylem vessel cells (in angiosperms) and tracheid cells (in ferns and gymnosperms). These cells are commonly characterized by secondary cell wall (SCW) formation and programmed cell death (PCD), which increase the efficiency of water

Identification and expression of the chloroplast clpP gene in the conifer Pinus contorta.

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Ingia / Ingia

The clpP gene from the conifer Pinus contorta was identified and isolated from a chloroplast genomic library by heterologous hybridisation to the second exon of the chloroplast clpP gene in tobacco. DNA sequencing of two overlapping clones revealed an uninterrupted 615 bp open-reading frame with 41

Protective Effects of Pinus halepensis Bark Extract and Nicotine on Cigarette Smoke-induced Oxidative Stress in Keratinocytes

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Ingia / Ingia

Background/aim: Cigarette smoke (CS) is a major environmental health threat. The oxidative stress induced by CS on keratinocytes and the possible protective effect of nicotine, its receptor inhibitors, and Pinus halepensis bark extract in

Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum.

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Ingia / Ingia

We isolated the 5' flanking region of a gene for phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) from Pinus taeda, PtaPAL. To investigate the tissue-specific expression of the PtaPAL promoter, histochemical assay of GUS activity was performed using the transgenic tobacco expressing the PtaPAL

A cerato-platanin-like protein HaCPL2 from Heterobasidion annosum sensu stricto induces cell death in Nicotiana tabacum and Pinus sylvestris.

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Ingia / Ingia

The cerato-platanin family is a group of small secreted cysteine-rich proteins exclusive for filamentous fungi. They have been shown to be involved in the interactions between fungi and plants. Functional characterization of members from this family has been performed mainly in Ascomycota, except

Towards male sterility in Pinus radiata--a stilbene synthase approach to genetically engineer nuclear male sterility.

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Ingia / Ingia

A male cone-specific promoter from Pinus radiata D. Don (radiata pine) was used to express a stilbene synthase gene (STS) in anthers of transgenic Nicotiana tabacum plants, resulting in complete male sterility in 70% of transformed plants. Three plants were 98%-99.9% male sterile, as evidenced by

Isolation of 4-coumarate Co-A ligase gene promoter from loblolly pine (Pinus taeda) and characterization of tissue-specific activity in transgenic tobacco.

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Ingia / Ingia

We characterized promoter activity of a phenylpropanoid biosynthetic gene encoding 4-coumarate Co-A ligase (4CL), Pta4Clalpha, from Pinus taeda. Histochemical- and quantitative assays of GUS expression in the vascular tissue were performed using transgenic tobacco plants expressing promoter-GUS

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