plastoquinone 9/spinacia

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13 matokeo

Synthesis of plastoquinone-9 and phytylplastoquinone from homogentisate in lettuce chloroplasts.

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Ingia / Ingia

Chloroform-soluble extracts of unpurified chloroplast preparations of lettuce, pea and spinach and of class I lettuce chloroplasts that have been incubated in the light with [methylene-3H]homogentisate contain 3H-labelled plastoquinones-9 and -8 (minor homologue), 2-demethyplastoquinones-9 and -8

Catalytic reactions of the homogentisate prenyl transferase involved in plastoquinone-9 biosynthesis.

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Ingia / Ingia

Homogentisate solanesyl transferase (HST) catalyzes the prenylation and decarboxylation of homogentisate to form 2-methyl-6-solanesyl-1,4-benzoquinol, the first intermediate in plastoquinone-9 biosynthesis. In vitro, HST from Spinacia oleracea L., Arabidopsis thaliana, and Chlamydomonas reinhardtii

Investigation of the plastoquinone pool size and fluorescence quenching in thylakoid membranes and Photosystem II (PS II) membrane fragments.

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Ingia / Ingia

The efficiency of oxidized endogenous plastoquinone-9 (PQ-9) as a non-photochemical quencher of chlorophyll fluorescence has been analyzed in spinach thylakoids and PS II membrane fragments isolated by Triton X-100 fractionation of grana stacks. The following results were obtained: (a) After

Reconstitution of the endogenous plastoquinone pool in photosystem II (PS II) membrane fragments, inside-out-vesicles, and PS II core complexes from spinach.

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Ingia / Ingia

The possibility of reconstituting a functionally competent endogenous plastoquinone pool in photosystem II (PS II) membrane fragments, inside-out-vesicles (ISO-vesicles), and PS II core complexes was analyzed by measuring (i) the characteristic period four oscillation of the oxygen yield due to

Kinetics of S2 and S3 reduction by tyrosine Y(D) and other endogenous donors as a function of temperature in spinach PS II membrane fragments with a reconstituted plastoquinone pool.

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Ingia / Ingia

The characteristic period four oscillation patterns of oxygen evolution induced by a train of single-turnover flashes were measured as a function of temperature in dark-adapted photosystem II (PS II) membrane fragments that were reconstituted with native plastoquinone-9 (PQ-9) by a recently

The formation of homogentisate in the biosynthesis of tocopherol and plastoquinone in spinach chloroplasts.

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Ingia / Ingia

Homogentisate is the precursor in the biosynthesis of α-tocopherol and plastoquinone-9 in chloroplasts. It is formed of 4-hydroxyphenylpyruvate of the shikimate pathway by the 4-hydroxyphenylpyruvate dioxygenase. In experiments with spinach the dioxygenase was shown to be localized predominatedly in

Localization and synthesis of prenylquinones in isolated outer and inner envelope membranes from spinach chloroplasts.

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Ingia / Ingia

The prenylquinone content and biosynthetic capabilities of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts were analyzed. Both envelope membranes contain prenylquinones, and in almost similar amounts (on a protein basis). However, the outer envelope

Correlation between protein flexibility and electron transfer from QA-* to QB in PSII membrane fragments from spinach.

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Ingia / Ingia

To analyze a possible correlation between the extent of QA-* reoxidation and protein dynamics, fluorometric and Mössbauer spectroscopic measurements were performed in photosystem II membrane fragments from spinach. Numerical evaluation of the flash-induced change of the normalized fluorescence

Localization of prenylquinones in the envelope of spinach chloroplasts.

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Ingia / Ingia

The isolated and purified chloroplast envelope of spinach leaves contains, besides carotenoids, several prenylquinones as basic constituents: plastoquinone-9, phylloquinone K1, alpha-tocoquinone and the chromanol, alpha-tocopherol. The relative quinone and carotenoid composition of the envelope

L protein, encoded by psbL, restores normal functioning of the primary quinone acceptor, QA, in isolated D1/D2/CP47/Cytb-559/I photosystem II reaction center core complex.

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Ingia / Ingia

Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted

A Study of Plastoquinones in Photochemical Reactions in the Chloroplasts of Euglena gracilis Strain Z.

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Ingia / Ingia

Plastoquinone-9 (PQ-9) was isolated from the chloroplasts of Euglena gracilis Strain Z and spinach. The functional involvement and the structural specificity of PQ-9 in photochemical reactions was investigated in the isolated chloroplasts of Euglena gracilis. It was found that PQ-9 was required for

Scavenging of superoxide generated in photosystem I by plastoquinol and other prenyllipids in thylakoid membranes.

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Ingia / Ingia

We have examined scavenging of a superoxide by various prenyllipids occurring in thylakoid membranes, such as plastoquinone-9, alpha-tocopherolquinone, their reduced forms, and alpha-tocopherol, measuring oxygen uptake in hexane-extracted and untreated spinach thylakoids with a fast oxygen electrode

Role of PSII-L protein (psbL gene product) on the electron transfer in photosystem II complex. 1. Over-production of wild-type and mutant versions of PSII-L protein and reconstitution into the PSII core complex.

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Ingia / Ingia

To establish a system for over-production of PSII-L protein which is a component of photosystem II (PSII) complex, a plasmid designated as pMAL-psbL was constructed and expressed in Escherichia coli JM109. A fusion protein of PSII-L and maltose-binding proteins (53 kDa on SDS-PAGE) was accumulated

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