68Ga-BNOTA-PRGD2 PET/CT in Evaluation of Stroke
Anahtar kelimeler
Öz
Açıklama
Integrin αvβ3 is an important member of this receptor family and expressed preferentially on regenerative vascular endothelial cells, but not or very low on the quiescent vessel cells and other normal cells. The αvβ3 integrin is a key mediator of angiogenesis and thus may be an important diagnostic and therapeutic target associated with cerebrovascular repair processes after stroke.
The tri-peptide sequence of arginine-glycine-aspartic acid (RGD) can specifically bind to the integrin αvβ3 receptor. Accordingly, a variety of radiolabeled RGD-based peptides have been developed for non-invasive imaging of integrin αvβ3 expression via positron emission tomography (PET) or single photon emission computed tomography (SPECT) to monitor the angiogenesis in clinical Oncology and Cardiology. In Neurology, angiogenesis imaging based on integrin αvβ3 receptor has not been found in clinical trials, but preclinical animal studies showed it had great potential for clinical translation. Recently, series of RGD dimeric peptides with PEG linkers have been studied. The new types of RGD peptides showed much higher in vitro integrin αvβ3 binding affinity than the single RGD tri-peptide sequence. As a representative, 68Ga-BNOTA-PRGD2 could be easily prepared and exhibited excellent in vivo behavior in animal models and also tumor or myocardial infarction patients. No adverse reactions are observed in animal models or patients to date.
For the further interests in clinical translation of 68Ga-BNOTA-PRGD2, an open-label brain PET/CT study was designed to investigate diagnostic performance of 68Ga-BNOTA-PRGD2 in stroke patients in convalescence. A single dose of nearly 111 MBq 68Ga-BNOTA-PRGD2 ( ≤ 40 µg BNOTA-PRGD2) will be intravenously injected into the patients. Visual and semiquantitative method will be used to assess the PET/CT images. Changes of brain 18F-FDG PET/CT, enhanced brain MRI or CT, and any adverse events will be collected from the patients.
Tarih
Son Doğrulandı: | 10/31/2014 |
İlk Gönderilen: | 07/31/2012 |
Tahmini Kayıt Gönderildi: | 07/31/2012 |
İlk Gönderilen: | 08/02/2012 |
Son Güncelleme Gönderildi: | 04/04/2017 |
Son Güncelleme Gönderildi: | 04/06/2017 |
Fiili Çalışma Başlangıç Tarihi: | 01/31/2012 |
Tahmini Birincil Tamamlanma Tarihi: | 11/30/2017 |
Tahmini Çalışma Tamamlanma Tarihi: | 11/30/2017 |
Durum veya hastalık
Müdahale / tedavi
Drug: Brain 68Ga-BNOTA-PRGD2
Evre
Kol Grupları
Kol | Müdahale / tedavi |
---|---|
Experimental: Brain 68Ga-BNOTA-PRGD2 We will perform brain 68Ga-BNOTA-PRGD2 PET/CT on stroke patients to determine its value. | Drug: Brain 68Ga-BNOTA-PRGD2 Intravenous injection of one dosage of 111MBq 68Ga-BNOTA-PRGD2 solution. Tracer doses of 68Ga-BNOTA-PRGD2 will be used to image angiogenesis of cerebral infarction areas by Positron Emission Tomography / computed tomography (PET/CT) |
Uygunluk kriterleri
Çalışmaya Uygun Yaşlar | 30 Years İçin 30 Years |
Çalışmaya Uygun Cinsiyetler | All |
Sağlıklı Gönüllüleri Kabul Ediyor | Evet |
Kriterler | Inclusion Criteria: stroke patients in convalescence: - Males and females, ≥30 years old - Confirmed cerebral infarction diagnosis - With brain CT or MRI scans Exclusion Criteria: - Have cerebral tumors or other kinds of cerebral diseases - Females planning to bear a child recently or with childbearing potential - Renal function: serum creatinine >3.0 mg/dL (270 μM/L) - Liver function: any hepatic enzyme level more than 5 times upper limit of normal. - Known severe allergy or hypersensitivity to IV radiographic contrast. - Patients not able to enter the bore of the PET/CT scanner. - Inability to lie still for the entire imaging time because of cough, pain, etc. - Inability to complete the needed examinations due to severe claustrophobia, radiation phobia, etc. - Concurrent severe and/or uncontrolled and/or unstable other medical disease that, in the opinion of the Investigator, may significantly interfere with study compliance. |
Sonuç
Birincil Sonuç Ölçütleri
1. Visual and semiquantitative assessment (Standardized Uptake Values = SUVs) of cerebral infarction region, SUV ratios (SUVinfarction/SUV contralateral) [1 year]
İkincil Sonuç Ölçütleri
1. Adverse events collection [1 year]