HMGB1 Release From Hemorrhagic Shock Patients

Sadece kayıtlı kullanıcılar makaleleri çevirebilir

Giriş yapmak kayıt olmak

Bağlantı panoya kaydedilir

DurumTamamlandı

Sponsorlar

Xingui Dai

KLINIK ÇALIŞMA: NCT03535441

BioSeek: NCT03535441

Anahtar kelimeler

Öz

It is reported that high mobility group box 1 (HMGB1), a non-histone nuclear protein, can serve as an alarmin with damage associated molecular patterns to activate immune responses in the early stages of hemorrhagic shock (HS). However, the origin of HMGB1 and how it is released following HS is poorly understood. In this study, we teased out this mechanism. We try to record the concentration of serum HMGB1 protein following HS in clinical patients.

Açıklama

The study was approved by the First People's Hospital of Chenzhou, Hunan, P.R. China. Consent was obtained from patients in their hospital course. Eighteen patients were enrolled for each group. In addition, Acute Physiological and Chronic Health Evaluation II (APACHE II) score was used to evaluate the severity and morbidity of HS patients Serial blood samples were collected at indicated time points on hospital admission for HS patients (0, 2, 4, 8, 24, and 72 h following HS). Levels of HMGB1 were detected by ELISA according to the kit manufacturer's instructions.

Tarih

Son Doğrulandı:	04/30/2018
İlk Gönderilen:	05/10/2018
Tahmini Kayıt Gönderildi:	05/13/2018
İlk Gönderilen:	05/23/2018
Son Güncelleme Gönderildi:	05/13/2018
Son Güncelleme Gönderildi:	05/23/2018
Fiili Çalışma Başlangıç Tarihi:	05/16/2017
Tahmini Birincil Tamamlanma Tarihi:	04/10/2018
Tahmini Çalışma Tamamlanma Tarihi:	05/10/2018

Durum veya hastalık

Intensive Care Unit

Hemorrhagic Shock

Adult Disease

Evre

Kol Grupları

Kol	Müdahale / tedavi
voluteer group No treatment, only blood sample collection
hemorrhagic shock group HS was defined as out-of-hospital systolic blood pressure (SBP) of 70 mmHg or less or SBP ranging 71 to 90 mmHg with a heart rate of 108 beats/min or more. Exclusion criteria were pregnancy, <15 years old, more than 2,000 mL of intravenous fluids or blood before enrollment, hypothermia, drowning, asphyxia, burns, isolated penetrating head injury, time of call received by dispatch to study intervention longer than 4 h, known prisoners, and transfer from another hospital

Kol

Müdahale / tedavi

voluteer group

No treatment, only blood sample collection

hemorrhagic shock group

HS was defined as out-of-hospital systolic blood pressure (SBP) of 70 mmHg or less or SBP ranging 71 to 90 mmHg with a heart rate of 108 beats/min or more. Exclusion criteria were pregnancy, <15 years old, more than 2,000 mL of intravenous fluids or blood before enrollment, hypothermia, drowning, asphyxia, burns, isolated penetrating head injury, time of call received by dispatch to study intervention longer than 4 h, known prisoners, and transfer from another hospital

Uygunluk kriterleri

Çalışmaya Uygun Yaşlar	18 Years İçin 18 Years
Çalışmaya Uygun Cinsiyetler	All
Örnekleme yöntemi	Probability Sample
Sağlıklı Gönüllüleri Kabul Ediyor	Hayır
Kriterler	Inclusion Criteria: Hemorrhagic shock (HS) was defined as out-of-hospital systolic blood pressure (SBP) of 70 mmHg or less or SBP ranging 71 to 90 mmHg with a heart rate of 108 beats/min or more. Exclusion Criteria: pregnancy, <18 years old, more than 2,000 mL of intravenous fluids or blood before enrollment, hypothermia, drowning, asphyxia, burns, isolated penetrating head injury, time of call received by dispatch to study intervention longer than 4 h, known prisoners, and transfer from another hospital

Sonuç

Birincil Sonuç Ölçütleri

1. serum HMGB1 concentration [24 hours following hemorrhagic shock]

the concentration of serum HMGB1 were progressively increased following Hemorrhagic shock