Dopaminergic Receptors in Sydenham's Chorea
Anahtar kelimeler
Öz
Açıklama
Participants:
The investigators will enroll participants with acute Sydenham's chorea (aSC) and age-matched controls with streptococcal tonsillitis (ST) within the past 4 months and healthy children (HC) with no a history of a strep infection in the past 6 months and negative throat culture. Informed consent will be obtained from participants and/ or parents. Children with SC will be evaluated for SC severity using the USCRS. The investigators will record clinical data regarding gender, age, duration symptoms, and medical treatments. Controls will be examined by referring physician to rule out chorea. Sera samples from all participants will be stored at -70°C and shipped to the microbiology and immunology lab, University of Oklahoma Health Sciences Center (OHSC) on dry ice.
Laboratory testing:
Immunophenotyping for anti-D1R, anti-D2R, anti- LG and anti-tubulin titers and signaling studies will be performed at the OUHSC microbiology and immunology laboratory.
Autoantibody titers. Sera will be assayed for reactivity with the dopamine D1 and D2 receptors (Human dopamine D1 and D2 receptor membrane antigens, Perkin Elmer-Membrane Target Systems) in the direct ELISA and assays which include the ELISA competitive inhibition. Anti- LG and anti-tubulin titers will be tested as well.
Statistical Analysis:
Time will be categorized in order to model non-linear associations among the groups. Means will be compared among groups using an ANOVA model that included main effects for the time, participant diagnosis and the interaction between participant diagnosis and time. Modeling assumptions will be evaluated using residual plots. A natural log transformation of the antibody measure will be used when there was evidence of non-constant variance across the groups. If a significant interaction between time and participant group will be found, time trends will be summarized separately within each participant group. Post-hoc pair-wise comparisons will be made using Tukey's method to control the type I error rate. A two-sided 0.05 alpha level will be used to define statistical significance. Analyses will be performed using SAS (SAS Institute Inc., SAS v9.4, Cary, NC: SAS Institute Inc.).
Tarih
Son Doğrulandı: | 07/31/2019 |
İlk Gönderilen: | 01/01/2019 |
Tahmini Kayıt Gönderildi: | 09/07/2019 |
İlk Gönderilen: | 09/09/2019 |
Son Güncelleme Gönderildi: | 09/07/2019 |
Son Güncelleme Gönderildi: | 09/09/2019 |
Fiili Çalışma Başlangıç Tarihi: | 06/30/2016 |
Tahmini Birincil Tamamlanma Tarihi: | 12/29/2019 |
Tahmini Çalışma Tamamlanma Tarihi: | 12/29/2021 |
Durum veya hastalık
Müdahale / tedavi
Diagnostic Test: Antibody detection
Evre
Kol Grupları
Kol | Müdahale / tedavi |
---|---|
Sydenam Chorea (SC) individuals with SC | |
tonsilitis children with tonsilitis in the past 3 months | |
control children wit no tonsilitis |
Uygunluk kriterleri
Çalışmaya Uygun Yaşlar | 2 Years İçin 2 Years |
Çalışmaya Uygun Cinsiyetler | All |
Örnekleme yöntemi | Probability Sample |
Sağlıklı Gönüllüleri Kabul Ediyor | Evet |
Kriterler | Inclusion Criteria: - sydenham chora OR strep tonsilitiscontrol without strep tonsilitis OR Exclusion Criteria: - neurological disrder autoimmune disorder |
Sonuç
Birincil Sonuç Ölçütleri
1. Antibody titers [Once at time of initial visit within the first 4 months from onset]
2. UFMG Sydenham's Chorea Rating Scale [Upon presentation when symptomatic, usually within 4 months of onset]