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isocoumarin/lösemi
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Selective isocoumarin serine protease inhibitors block RNK-16 lymphocyte granule-mediated cytolysis.
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Granules that are potently cytolytic in vitro can be obtained from cytotoxic lymphocytes that kill virally infected cells and tumor cells. These granules contain pore-forming proteins and several serine proteases. Here we indicate that at least two different proteases participate in the lysis
New substituted isocoumarins and Dihydroisocoumarins and their cytotoxic activities.
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New isocoumarins were prepared in an efficient way from 2-iodobenzoic acid derivatives and hept-1-yne in a Sonogashira reaction, followed by spontaneous cyclization. Catalytic hydrogenation gave the corresponding dihydroisocoumarins. A 4-chloroisocoumarin was prepared on an alternative pathway. Some
A new isocoumarin from Hypericum annulatum.
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A new isocoumarin, annulatomarin (1), together with the known physcion and beta-sitosterol were isolated from the aerial parts of Hypericum annulatum. The structure of the new compound was established as 6,8-dihydroxy-7-methoxy-3-phenyl-3,4-dihydro-1H-isochromen-1-one on the basis of detailed
Induction of apoptosis by T-2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells.
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Based on the DNA fragmentation profile in gel electrophoresis and the morphological changes in electron microscopy, the induction of apoptotic nuclear changes by mycotoxins and other microbial products, in total 31 chemicals, was investigated in HL-60 human promyelotic leukemia cells, along with the
Localization, implications for function, and gene expression of chymotrypsin-like proteinases of cytotoxic RNK-16 lymphocytes.
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Rat RNK-16 leukemia cells kill YAC-1, which are the cells lysed by rodent natural killer lymphocytes. We found chymotrypsin-like proteinase ('chymase') activity in the RNK-16 dense granules that also contain cytolytic activity. The chymase activity hydrolyzed the thiobenzyl peptide substrate
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