Described is a nucleic acid coding for a protein called "cyplasin" that shows a preferential toxicity to autonomously growing mammalian cells. Cell death induced by this protein differs from both apoptosis and necrosis. An intracellular cell death which occurs when recombinantly preparing cyplasin in cell cultures can be avoided by removal of the secretion signal in the cyplasin sequence. This modification makes it possible to express the cyplasin in a mammalian cell culture which is preferable with regard to the glycosylation pattern of the obtained protein. Thus, the present invention also relates to a method of recombinantly producing a protein in eukaryotic cells, preferably mammalian cells, which is cytotoxic for said cells when applied externally.

Cyplasin Biomedical, Ltd.

Cytotoxic cyplasin of the sea hare, aplysia punctata, cdna cloning and expression of bioreactive recombinants

The present invention relates to genetic markers for discrimination and detection of viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same, and more particularly to a method for discriminating or detecting viruses causing infectious aquatic organism diseases, the method comprising: selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish would be infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.

National Institute of Fisheries Science

NATIONAL INSTITUTE OF FISHERIES SCIENCE

Genetic markers for discrimination and detection of bercoiver tk region on koi herpesvirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same

Disclosed are interactions between different betanodaviruses during infection of cells "in vitro" and in European sea bass. More specifically, fish administered striped jack nervous necrosis viruses (SJNNV), which are then exposed to red-spotted grouper nervous necrosis viruses (RGNNV), have fewer symptoms of disease associated with RGNNV and/or increased survival as compared to fish not administered SJNNV prior to exposure to RGNNV.

sea/nekroz

Cytotoxic cyplasin of the sea hare, aplysia punctata, cdna cloning and expression of bioreactive recombinants

Genetic markers for discrimination and detection of bercoiver tk region on koi herpesvirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same

Interactions of betanodaviruses in infection

Bilim tarafından desteklenen en eksiksiz şifalı otlar veritabanı