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UPLC-ESI-MS/MS and HPTLC Method for Quantitative Estimation of Cytotoxic Glycosides and Aglycone in Bioactivity Guided Fractions of Solanum nigrum L.

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Solanum nigrum L., is traditionally used for the management of the various liver disorders. Investigating the effect of polarity based fractionation of S. nigrum for its hepatoprotective effect on Hep G2 cells in vitro to provide base of its activity by quantifying in steroidal glycosides

Liquid chromatographic determination of alpha-solasonine in frozen green peas as an indicator of the presence of nightshade berries.

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Nightshade berries containing glycoalkaloids can be a contaminant in green peas. Methodology was developed to detect this contamination. The glycoalkaloid alpha-solasonine was extracted from frozen green peas with 1% (v/v) acetic acid, cleaned up on a C18 cartridge, and determined by liquid

Bioactive steroidal alkaloids from the fruits of Solanum nigrum.

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The investigation of the fruits of Solanum nigrum led to the isolation of four previously undescribed steroidal alkaloids, named solanine A, 7α-OH khasianine, 7α-OH solamargine and 7α-OH solasonine, together with six known ones. The structures of the isolated compounds were elucidated unambiguously

Steroidal glycoalkaloids from Solanum nigrum target cytoskeletal proteins: an in silico analysis.

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Background

Solanum nigrum (black nightshade; S. nigrum), a member of family Solanaceae, has been endowed with a heterogeneous array of secondary metabolites of which the steroidal glycoalkaloids (SGAs) and steroidal saponins (SS) have vast potential to serve as

[Study on HPLC Fingerprint of Solanum nigrum Fruit].

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To establish the HPLC fingerprint method of Solanum nigrum fruit for its identification and quality evaluation. The analysis was carried out by Agilent ZORBAX SB-C18column( 150 mm × 4. 6 mm,5 μm),and eluted with mobile phase containing of acetonitrile-0. 3% phosphoric acid in a gradient mode. The

[Simultaneous determination of three steroidal alkaloids from Solanum Nigrum by RP-HPLC].

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OBJECTIVE A new method for simultaneous determination of solasonine (1), solamargine (2) and khasianine (3) in Solanum Nigrum by reversed-phase HPLC was developed. METHODS The samples were separated at 30 degrees C on Agilent Zorbax SB C18 (4.6 mm x 150 mm, 5 microm) column with

The application of an aqueous two-phase system combined with ultrasonic cell disruption extraction and HPLC in the simultaneous separation and analysis of solanine and Solanum nigrum polysaccharide from Solanum nigrum unripe fruit.

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An aqueous two-phase system was used in conjunction with ultrasonic cell disruption to extract and separate solanine (mainly solasonine and solamargine) and Solanum nigrum polysaccharide from Solanum nigrum unripe fruit. The optimized conditions of the present study were determined by a

Purification, antitumor activity in vitro of steroidal glycoalkaloids from black nightshade (Solanum nigrum L.).

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Six steroidal glycoalkaloids (1-6) were isolated and purified from Solanum nigrum L. (SNL) by acid extraction and alkaline precipitation, various chromatographic techniques, and their structures were elucidated by spectroscopic data. Antitumor activity, structure-activity and its molecular mechanism

In vivo antimalarial activities of glycoalkaloids isolated from Solanaceae plants.

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BACKGROUND Malaria is one of the most common and serious protozoan tropical diseases. Multi-drug resistance remains pervasive, necessitating the continuous development of new antimalarial agents. OBJECTIVE Many glycosides, such as triterpenoid saponins, were shown to have antimalarial activity

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