Cohort of operatively treated patients with acute Achilles tendon rupture.

Biopsies from acutely ruptured Achilles tendonds will be taken, Two biopsies from the ruptured part of the tendon and two biopsies from the healthy tissue of the same tendon proximal to the rupture, as a control.

Achilles Tendon Rupture

Department of Orthopedic Surgery, Copenhagen University Hospital, Hvidovre

Detection of Bacterial DNA and Collagen Metabolism in Acutely Ruptured Achilles Tendons

14C concentration in biopsies from the ruptured part of the tendons and in the control biopsies proximal to the rupture are compared to the known historical values of atmospheric 14C to estimate the rate of collagen turnover. The values for turnover in the biopsies will be compared to the known rates of turnover in healthy tendons.

2H isotope enrichment in the biopsies from the ruptured part of the tendons and in the control biopsies proximal to the rupture.

The enrichment of the 15N marked proline tracer measured in the biopsies from the ruptured part of the tendons and in the control biopsies and compared to the enrichment of 15N marked proline in the blood. Based on that, the fractional synthesis rate of protein at the time of surgery is calculated.

Presence of bacterial DNA in the biopsies from the ruptured part of the tendons and in the control biopsies proximal to the rupture detected by 16s rDNA PCR

rheumatic diseases/пролін

Detection of Bacterial DNA and Collagen Metabolism in Acutely Ruptured Achilles Tendons

Найповніша база даних про лікарські трави, підкріплена наукою