Age/sex matched control participants, not suffering from inflammatory bowel disease or any other chronic inflammatory disease

Patients with active or remissive inflammatory bowel disease

Inflammatory Bowel Disease

Crohn Disease

Ulcerative Colitis

Great Ormond Street Hospital

Vascular Dysfunction in Paediatric IBD

Investigation of Vascular Endothelial Dysfunction, Thromboembolism and Structural Arterial Disease in Paediatric and Adolescent Inflammatory Bowel Disease (IBD)

Pulse wave velocity is a measure of arterial stiffness, and correlates with cardiovascular events and all-cause mortality.

Plasma microparticles will be isolated and enumerated by flow cytometry

The thickness of the carotid tunica intima and tunica media will be assessed by ultrasound. This thickness correlates with atherosclerosis.

Plasma microparticles will be isolated and their pro-thrombotic potential will be assessed by calibrated automated thrombogram (CAT).

Circulating endothelial cells enumerated from whole blood, as a marker for endothelial dysfunction.

Great Ormond Street Hospital for Children NHS Foundation Trust

National Institute for Health Research, United Kingdom

Subject assigned to this arm will be transfused with washed RBC

Subjects assigned to this arm will be transfused with unwashed RBC

Subject assigned to this arm will be transfused with washed RBC
Washed RBC: There is no pre-set dosage, frequency and duration for transfusion with washed RBC. It all depends on the conditions of patients during and after surgery. As circumstances arise, the physician will request needed washed RBC for the subject.

Transfusion With Washed RBC

Subjects assigned to this arm will be transfused with unwashed RBC
Unwashed RBC: There is no pre-set dosage, frequency and duration for transfusion with unwashed RBC. It all depends on the conditions of patients during and after surgery. As circumstances arise, the physician will request needed unwashed RBC for the subject.

Transfusion With Unwashed RBC

Total

Sex: Female, Male

Ethnicity (NIH/OMB)

Race (NIH/OMB)

Region of Enrollment

Cd41+ platelet microparticles is a specific biomarker for platelet activation

CD41+ platelet microparticles

Cd235a+ red cell microparticles are a specific marker for red cell storage lesions. They may reflect the conditions of transfused RBC in circulation.

CD235a+ red cell microparticles

CD62E+ endothelial microparticles are a specific marker for endothelial activation and apoptosis.

CD62E+ endothelial microparticles

Annexin V+ microparticles are a marker for non-specific cell activation and apoptosis.

Annexin V+ microparticles

The number of participants who expired during hospital stay after CABG surgery

Subjects assigned to this arm were transfused with washed RBC
Washed RBC: There is no pre-set dosage, frequency, or duration for transfusion with washed RBC. It all depends on the conditions of the patient during and after surgery. As circumstances arise, the physician will request needed washed RBC for the subject.

Subjects assigned to this arm were transfused with unwashed RBC
Unwashed RBC: There is no pre-set dosage, frequency, or duration for transfusion with unwashed RBC. It all depends on the conditions of the patient during and after surgery. As circumstances arise, the physician will request needed unwashed RBC for the subject.

In Hospital Mortality

Number participants who expired within one year after CABG surgery

Subjects assigned to this arm were transfused with washed RBC
Washed RBC: There is no pre-set dosage, frequency,or duration for transfusion with washed RBC. It all depends on the conditions of the patient during and after surgery. As circumstances arise, the physician will request needed washed RBC for the subject.

One-year Mortality

Comparison of the two groups with respect to occurrence or not of at least one SAE including sepsis, respiratory failure, multi-organ failure, anaphylactic shock, transfusion-related acute lung injury, MI, stroke, cardiac arrest.

Occurrence of at Least One Serious Adverse Event (SAE)

Difference in levels of circulating CD41+ platelet microparticles between at pre-surgery and at 1hour post-surgery, i.e. level at 1hour post-surgery - level at pre-surgery

Difference in Levels of Circulating CD41+ Platelet-derived Microparticles 1 Hour Post-surgery

Difference in levels of circulating Annexin V+ microparticles between at pre-surgery and 1 hour post-surgery, i.e. level of Annexin V+ microparticles at 1 hour post-surgery - level of Annexin V+ microparticles at pre-surgery

Difference in Levels of Circulating Annexin V+ Microparticles 1 Hour Post- Surgery

Difference in levels of circulating CD62E+ endothelial microparticles between 1 hour post-surgery and at pre-surgery, i.e. level at 1 hour post-surgery - level at pre-surgery

Difference in Levels of Circulating CD62E+ Endothelial Microparticles 1 Hour Post-surgery

Difference in levels of circulating CD235a+ red cell microparticles between at 1 hour post-surgery and at pre-surgery, i.e. levels at 1 hour post-surgery - level at pre-surgery

Difference in Levels of Circulating CD235a+ Red Cell Microparticles 1 Hour Post-surgery

All clinical non-serious adverse events reported in the clinical chart were recorded in the study's data files. This type of events, defined as Non-Serious Adverse Events or just Adverse Events (AEs) were categorized into 5 groups: (1) Cardiovascular / respiratory; (2) renal; (3) neurologic (CNS); (4) infections; and (5) other. For each participant, the outcome measure was defined as the number of AE's he or she experienced during his/her hospital stay.

Each Participant's Number of Non-serious Adverse Events

Overall Study

Wenche Jy, PhD, Research Associate Professor, Principal Investigator

Coronary Artery Bypass

There is no pre-set dosage, frequency and duration for transfusion with washed RBC. It all depends on the conditions of patients during and after surgery. As circumstances arise, the physician will request needed washed RBC for the subject.

Washed RBC

There is no pre-set dosage, frequency and duration for transfusion with unwashed RBC. It all depends on the conditions of patients during and after surgery. As circumstances arise, the physician will request needed unwashed RBC for the subject.

ulex/запалення

Vascular Dysfunction in Paediatric IBD

Microparticles in Stored RBC as Potential Mediators of Transfusion Complications

Найповніша база даних про лікарські трави, підкріплена наукою