CCK1R Function in Patient Leukocytes
关键词
抽象
描述
Subjects are volunteers who provide prospective informed consent to be entered into the Sangre Por Salud Biobank at Mountain Park Medical Center. These include men and women age 18-85 of Hispanic/Latino descent, who are not pregnant or have given birth in the previous 12 months, or who are nursing, and who have not been diagnosed with cancer in the previous three years. Two groups of patients are included, representing those who have not been diagnosed as having diabetes, and those with diabetes. Subjects undergo entry physical examination and standard metabolic phenotyping, with the non-diabetics also undergoing a 2-hour glucose tolerance test. Blood samples are also banked and available for other studies, such as the one now being described.
For the current study, 5 ml of blood will be coded and delivered in an anonymized form. Buffy coat cells will be isolated and infected ex vivo with a viral construct including the wild type CCK1R tagged with a fluorescent tag. Cells will be placed in culture overnight and harvested for quantitation of receptor expression and determination of CCK responsiveness and cholesterol content. These data will be correlated with selected relevant clinical, biochemical, and morphometric data.
日期
最后验证: | 03/31/2017 |
首次提交: | 04/16/2017 |
提交的预估入学人数: | 04/18/2017 |
首次发布: | 04/19/2017 |
上次提交的更新: | 04/18/2017 |
最近更新发布: | 04/19/2017 |
实际学习开始日期: | 01/20/2015 |
预计主要完成日期: | 02/28/2017 |
预计完成日期: | 04/13/2017 |
状况或疾病
相
手臂组
臂 | 干预/治疗 |
---|---|
Sangre Por Salud cohort members Subjects entered in the Sangre Por Salud Biobank |
资格标准
有资格学习的年龄 | 18 Years 至 18 Years |
有资格学习的性别 | All |
取样方式 | Non-Probability Sample |
接受健康志愿者 | 是 |
标准 | Inclusion Criteria: - volunteers Exclusion Criteria: - Hispanic/Latino descent not pregnant, or given birth in the previous 12 months, or currently nursing not diagnosed with cancer in the past three years |
结果
主要结果指标
1. Total cellular cholesterol on leukocytes collected from the subjects [baseline]