Effect of Kale Consumption on Human Xenobiotic Metabolizing Enzymes
关键词
抽象
描述
Consumption of Brassica vegetables (which include broccoli, cabbage, and kale) is inversely associated with the incidence of several cancers, including cancers of the lung, stomach, liver, colon, rectum, breast, endometrium, and ovaries. Brassica vegetables are a good source of many nutrients, but the unique characteristic of Brassicas is their rich content of glucosinolates. Glucosinolates are sulfur-containing compounds that are converted to bioactive metabolites by a plant enzyme called myrosinase, which is released when the vesicles containing myrosinase are ruptured by chewing or cutting. These bioactive compounds are considered to be the active agent for cancer prevention. Their ability to reduce risk of cancer may derive in part from their ability to modulate foreign-substance metabolizing enzymes, which include enzymes called Phase I cytochrome P450s and Phase II enzymes.
The primary aim of this study is to investigate how daily consumption of kale influences foreign-substance metabolizing enzymes, which in turn may reduce cancer risk. Secondary aims of this study include measuring metabolism of kale nutrients, effect of kale consumption on fecal microbiota, and how kale consumption influences risk factors for cardiovascular disease.
日期
| 最后验证: | 08/31/2018 |
| 首次提交: | 02/21/2018 |
| 提交的预估入学人数: | 02/21/2018 |
| 首次发布: | 02/27/2018 |
| 上次提交的更新: | 09/10/2018 |
| 最近更新发布: | 09/11/2018 |
| 实际学习开始日期: | 04/17/2018 |
| 预计主要完成日期: | 07/31/2018 |
| 预计完成日期: | 07/31/2018 |
状况或疾病
干预/治疗
Other: Base diet
Other: Kale Treatment
相
手臂组
| 臂 | 干预/治疗 |
|---|---|
| Other: Base diet Subjects will consume a base diet prepared using traditional American foods with a macronutrient composition representative of a typical American diet. | Other: Base diet Base Diet |
| Other: Kale Treatment Subjects will consume 500 g of kale per 2000 kcal of food, split between breakfast and dinner, as a supplement to the base diet. | Other: Kale Treatment Base Diet plus Kale |
资格标准
| 有资格学习的年龄 | 21 Years 至 21 Years |
| 有资格学习的性别 | All |
| 接受健康志愿者 | 是 |
| 标准 | Inclusion Criteria: - 5 years cancer free - Not a tobacco product user - Blood glucose less than 126 mg/dL - Able to voluntarily agree to participate and sign an informed consent document Exclusion Criteria: - Brassica vegetable allergy or intolerance - use of oral contraceptives - Women who have given birth in the previous 12 months - Type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots - Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol, or Miradon (anisindione) - History of bariatric surgery or nutrient malabsorption disease - Pregnant, lactating, or intending to become pregnant during the study period - Crohn's disease or diverticulitis - Suspected or known strictures, fistulas or physiological/mechanical GI obstruction - Self-report of alcohol or substance abuse within the past 12 months and/or current acute treatment or rehabilitation program for these problems (long-term participation in Alcoholics Anonymous is not an exclusion) |
结果
主要结果指标
1. CYP1A2 activity will be analyzed [Day 7]
2. CYP1A2 activity will be analyzed [Day 14]
3. CYP1A2 activity will be analyzed [Day 42]
4. CYP1A2 activity will be analyzed [Day 49]
次要成果指标
1. The ability of fecal microbiota to metabolize glucosinolates will be determined [Days 14 and 49.]
2. Metabolites of Kale [On days 35 and 36]
3. Fecal microbiota will be analyzed for microbial DNA [Days 0, 14, 35, and 49]
4. UGT1A1 activity will be analyzed [On days 7, 14, 42, and 49]
5. Glutathione S-transferase alpha concentration [On days 7, 14, 42, and 49]
6. Total cholesterol [On days 0, 7, 14, 35, 42, and 49]
7. LDL cholesterol [On days 0, 7, 14, 35, 42, and 49]
8. HDL cholesterol [On days 0, 7, 14, 35, 42, and 49]
9. Triacylglycerides [On days 0, 7, 14, 35, 42, and 49]
10. Apolipoprotein A1 [On days 0, 7, 14, 35, 42, and 49]
11. Apolipoprotein A2 [On days 0, 7, 14, 35, 42, and 49]
12. Apolipoprotein B [On days 0, 7, 14, 35, 42, and 49]
13. Changes in gene expression [On days 0, 14, 35, and 49]
