Genomic Outcomes of Metformin
关键词
抽象
描述
The number of older adults is projected to increase dramatically by 2050. Aging-related diseases and conditions still seriously compromise the quality of life among most older adults. Several pharmaceutical agents, such as metformin, have been tested to extend lifespan and delay aging-related diseases and dysfunctions in mice. Metformin, a biguanide antidiabetic drug, reduces the risk for developing type-2 diabetes in persons at risk by over one-third with few adverse effects (e.g., gastrointestinal irritation). Metformin prevents type-2 diabetes primarily through decreasing hepatic glucose synthesis, as well as enhancing insulin sensitivity and increasing peripheral glucose uptake. The molecular mechanisms remain unclear, although a number of potential mechanism such as activation of AMP-activated protein kinase (AMPK) and inhibition of mitochondrial glycerophosphate dehydrogenase have been proposed. The fact that metformin treatment in persons with type-2 diabetes has been associated with reduced risk of other aging-related diseases and conditions, including cardiovascular disease, cancer and cognitive decline supports the possibility of the beneficial effects of metformin on healthy aging. It is imperative to capitalize on these leads to extend health span among older adults.
To translate animal findings to human intervention trials, appropriate aging biomarkers are needed. Methylomic and transcriptomic profiles in relevant cells may reflect molecular features that mediate effects of both genetic and environmental factors on aging-related functional decline and disease. The roles of monocytes have been implicated in development of many aging-related diseases such as cardiovascular disease, cancer and neurodegenerative disease. In a cross-sectional association study of 1,200 monocyte samples, we identified 1,794 age-associated methylation sites and 2,704 age-associated transcripts, which were over-represented in two networks (autophagy and oxidative phosphorylation) and suggestive of decline in those functions with age. Both autophagy and oxidative phosphorylation are considered as key contributors to the aging process, and their dysfunctions have been linked to aging-related diseases. Changes in these aging-related omic biomarkers may be early indicators of cellular damage or disruption that eventually leads to age-related dysfunctions. Assessment of these aging biomarkers in response to therapeutic intervention may also provide molecular insight for personalizing treatment. The investigators propose a pilot study to examine changes in aging-related omic profiles after 3 months of metformin treatment in 35 monocyte samples from older adults using a randomized, double-blind, placebo-controlled crossover study design.
Our overarching goal of the pilot study is to evaluate the utility of using the aging-related omic biomarkers as an indicator of pharmacologic responses in the anti-aging therapeutic intervention trials through the following specific aims. Although this pilot study does not have sufficient power to definitively test all the aims, it will provide essential preliminary data for developing a full scale research program.
- Aim 1A: To test the effects of the metformin treatment on transcriptomic profiles and related functional changes in human monocytes,
- Aim 1B: To explore the effects of the metformin treatment on methylomic profiles in human monocytes,
- Aim 2: To investigate the longitudinal relationship between transcriptional and functional changes in human monocytes during the metformin treatment and
- Aim 3: To test the effects of the metformin treatment on frailty and other aging-related physical and cognitive measures and investigate the longitudinal relationship between these changes and transcriptional changes.
A randomized, double-blind, placebo-controlled crossover trial in 30 participants using metformin and matching placebo will be used. In the absence of a treatment by sequence interaction effect, this design can increase study power for evaluating treatment effects by allowing each participant to be his/her own control. The period effect may be minimum because the primary outcomes, methylation and transcriptional measures, are relatively stable overtime.
日期
| 最后验证: | 09/30/2019 |
| 首次提交: | 10/26/2016 |
| 提交的预估入学人数: | 12/04/2016 |
| 首次发布: | 12/07/2016 |
| 上次提交的更新: | 10/24/2019 |
| 最近更新发布: | 11/05/2019 |
| : | 10/07/2019 |
| : | 10/07/2019 |
| : | 10/21/2019 |
| 实际学习开始日期: | 08/02/2016 |
| 预计主要完成日期: | 11/14/2018 |
| 预计完成日期: | 11/14/2018 |
状况或疾病
干预/治疗
Drug: Metformin
Drug: Placebo
相
手臂组
| 臂 | 干预/治疗 |
|---|---|
| Active Comparator: Metformin then Placebo Metformin dosing at 425, 850 and 1700 mg with GLUCOPHAGE® (metformin hydrochloride) Tablets. Treatment with metformin will be initiated at a dose of 425 mg (half pill) taken orally once a day at night for 7 days. After the week, the participant will be called to assess tolerance and will be asked to increase dose to one pill at night at a dose of 850 mg for one week. At the end of the second week, participants will be called again and if they tolerated the second dose, they will be asked to take two 850 mg pills, one in the morning and one at night, for a total dose of 1700 mg which is within the range of the usual effective dose of 1500 to 2000 mg/day for the remainder of the 3 months. Will then cross over to 3 months on placebo. | |
| Placebo Comparator: Placebo then Metormin Dietary Supplement: Placebo with Methylcellulose capsules. Treatment with placebo will be initiated at a dose of a half pill taken orally once a day at night for 7 days. After the week, the participant will be called to assess tolerance and will be asked to increase dose to one pill at night for one week. At the end of the second week, participants will be called again and if they tolerated the second dose, they will be asked to take two pills, one in the morning and one at night for the remainder of the 3 months. After 3 months on placebo, participants will then cross over to 3 months of metformin as described in the other arm. |
资格标准
| 有资格学习的年龄 | 65 Years 至 65 Years |
| 有资格学习的性别 | All |
| 接受健康志愿者 | 是 |
| 标准 | Inclusion Criteria: - Age 65 - 79 Must meet criteria from one or more of the following groups: Group 1 (Can have 1 or 2 of these, but not all 3) - History of coronary artery disease (MI/heart attack, stroke, heart failure, or peripheral artery disease) - Cancer, with no active treatment in the last year - MCI (MoCA >18<26 -inclusive of 1 point if <12 years of education Group 2 - Decline physical function (walking speed < 1 m/s) Group 3 (Either or both) - Abdominal obesity (>88cm women, >102cm men) AND hypertension (treated or resting blood pressure >140/90 - Abdominal obesity (>88cm women, >102cm men) AND hyperlipidemia (treated or fasting total cholesterol >240 English literacy Willing to provide informed consent Exclusion Criteria: - eGFR <45 - Type 2 diabetes (HbA1c>6.5) or type 1 diabetes - Any tobacco or nicotine product use in the past year - Low vitamin B12 Levels (< 300 pg/mL) - Self-reported severe difficulty or inability to walk 400m or climb 10 steps (from Q 2 and 19 on PAT-D) - Self-reported difficulty or inability to perform basic ADL functions (from Q 10, 13, 14, 16 on PAT-D) - Excessive alcohol use (>14 drinks/week) - Cancer requiring treatment in past year (except skin) - Dementia - diagnosed and/or MoCA score <18 - Parkinson's or other neurological disease - Chronic liver disease or cirrhosis - End stage renal disease or on dialysis - Rheumatic conditions (Rheumatoid arthritis, lupus, and any other autoimmune disease the -PI deems them to be ineligible for) - Thyroid problems the PI deems them to be ineligible for - Gout - Involved in another interventional study - Hemoglobin <8 or diagnosed with anemia - Recent unintentional weight change (+/- 10 lbs. in the last 12 months) - BMI <18.5 - Likely to not follow the protocol - PI deems unfit to participate - Already taking Metformin or any other drug intended to treat diabetes |
结果
主要结果指标
1. Change in eigengene scores [baseline, 12 weeks, 24 weeks]
