MMP-9 Expression in Permanent Mature Teeth With Symptomatic Pulpitis
关键词
抽象
描述
Introduction: Widely used diagnostic tests do not provide information to distinguish the pulp inflammation stage while improvement of materials, techniques and vital pulp therapy have received a wider acceptance in symptomatic pulpitis treatment. The aim of this clinical study was to compare MMP-9 expression in healthy and inflamed pulp and to assess the short-term outcome of coronal pulpotomy in mature permanent teeth with symptomatic pulpitis.
Methods: Patients diagnosed with symptomatic pulpitis were included in this clinical study. Coronal pulpotomy was performed using calcium-silicate based material and blood samples were taken. Initial and postoperative pain was recorded by Heft-Parker visual analog scale at 24 hours and 72 hours after the procedure. In control group teeth with healthy pulp were used. During follow-up visits after three and six-months, patients were examined clinically and radiographically. The ELISA kit was used to determine the levels of MMP-9 in inflamed and healthy coronal pulp tissue.
日期
| 最后验证: | 12/31/2018 |
| 首次提交: | 11/18/2018 |
| 提交的预估入学人数: | 01/13/2019 |
| 首次发布: | 01/16/2019 |
| 上次提交的更新: | 01/13/2019 |
| 最近更新发布: | 01/16/2019 |
| 实际学习开始日期: | 03/26/2017 |
| 预计主要完成日期: | 07/14/2017 |
| 预计完成日期: | 02/10/2018 |
状况或疾病
干预/治疗
Procedure: Coronal pulpotomy
相
手臂组
| 臂 | 干预/治疗 |
|---|---|
| Other: Control group - Healthy teeth Patients with clinically healthy third molar or premolar teeth with indications for extraction; no clinical signs of pulpitis, no caries, no indications for the replacement of an old filling that is 1 mm from the pulp space as determined on the radiograph; a normal response to the cold test, and no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied. | |
| Experimental: Test group - Teeth with pulpitis Patients with symptomatic pulpitis resulting from caries; tooth pain defined as sharp, dull, localized or diffuse; pain at night; a symptomatic tooth with a positive response to the cold test and lingering pain; intermittent or continuous episodes of spontaneous pain (with no external stimulus) that could last from a few minutes up to a few hours; no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied. |
资格标准
| 有资格学习的年龄 | 14 Years 至 14 Years |
| 有资格学习的性别 | All |
| 接受健康志愿者 | 是 |
| 标准 | Inclusion Criteria: - Teeth diagnosed with symptomatic pulpitis - Patients who did not use any NSAIDs before the treatment - Positive cold test of investigated teeth - Clinically dental caries in contact with pulp chamber - Permanent teeth with radiographically closed root apex Exclusion Criteria: - Patients who refuse to participate in the study - Medically compromised patients (with immunosuppressive/systemic diseases, patients on medications) - Teeth with periapical radiolucency or clinical signs of apical periodontitis - Periodontological compromised teeth (probing depth ≥4mm) - Internal/external root resorption in periapical radiograph - Pulp chamber and/or root canal calcification in periapical radiograph - Teeth with unrestorable crown - Teeth with a negative response to cold test |
结果
主要结果指标
1. MMP-9 amount measurement using ELISA kit [All samples were measured in one time (during 12 hours)]
