Natural Antioxidant Ice-cream, Oxidative Stress and Vascular Function
关键词
抽象
描述
14 healthy, non-smoking, volunteers (7 males ), aged between 20 and 40 years were enrolled. The study was randomized, placebo-controlled. After enrollment, 3 days before entering the study, all subjects performed an exercise stress test. They were then randomly allocated to the treatment sequence with 100 mg of natural anti-oxidant ice cream or milk chocolate ice-cream (control icecream) in a cross-over single-blind design. There was 1 week wash-out between 2 phases of the study.
Markers of oxidative stress (D-ROMs and H2O2); serum antioxidant status (FRAP), NO bioavailability (NOx), the endothelium flow-mediated vasodilation (FMD and the RHI by Endopat 2000) were evaluated in fasting condition at baseline (time 0) and 2 hours after the ingestion of the anti-oxidant or control ice-creams. The exercise stress test was performed before randomization and 2 hours after each ice-cream meal test. In both experiments, the ice-cream was consumed within 10 min.
Each subject was asked to refrain from any kind of medication, diet integrators, intensive physical activity and to avoid consumption of polyphenols rich foods, such cocoa-containing products, red wine, tea within the previously 2 weeks. All participants gave written informed consent, and the study was approved by the Medical Ethics Committee of La Sapienza University of Rome (Rif: 3727, Prot: 2325/15).
日期
最后验证: | 01/31/2016 |
首次提交: | 02/07/2016 |
提交的预估入学人数: | 02/11/2016 |
首次发布: | 02/17/2016 |
上次提交的更新: | 02/17/2016 |
最近更新发布: | 02/18/2016 |
实际学习开始日期: | 06/30/2015 |
预计主要完成日期: | 09/30/2015 |
预计完成日期: | 11/30/2015 |
状况或疾病
干预/治疗
Other: Antioxidant Ice-cream
Other: Control Ice-cream
相
手臂组
臂 | 干预/治疗 |
---|---|
Experimental: Antioxidant Ice-cream Natural antioxidant Ice-cream | Other: Antioxidant Ice-cream Narutal Antioxidant ice-cream |
Placebo Comparator: Control Ice-cream Natural control ice-cream | Other: Control Ice-cream Natural Control Ice-cream |
资格标准
有资格学习的年龄 | 20 Years 至 20 Years |
有资格学习的性别 | All |
接受健康志愿者 | 是 |
标准 | Inclusion Criteria: - healthy, non-smoking, volunteers Exclusion Criteria: - Assumption of any kind of medication, diet integrators, intensive physical activity and to avoid consumption of polyphenols rich foods, tea within the previously 2 weeks. |
结果
主要结果指标
1. Endothelial function [Acute 2 hours]